Gülbudak Harun, Ülger Seda Tezcan, Bozok Taylan, Aslan Gönül
Mersin University Faculty of Science, Department of Biology, Mersin, Turkey.
Mersin University Faculty of Medicine, Department of Medical Microbiology, Mersin, Turkey.
One Health. 2025 May 18;20:101079. doi: 10.1016/j.onehlt.2025.101079. eCollection 2025 Jun.
is among the most common intestinal helminths, especially in children. Mitochondrial 1 gene analyses revealed three genotypes (A, B, C). We aimed to evaluate the epidemiological characteristics and 1 genotypes of cases diagnosed at Mersin University Hospital. Between January 2017 and December 2023, 1496 samples were examined using the cellophane tape method, and 75 positives were identified. Epidemiological data were retrospectively analyzed. DNA was isolated from 30 positive samples via the phenol-chloroform method, and nested PCR products of the 1 gene were sequenced. Phylogenetic analyses were performed using GenBank sequences. In a sample of patients with gastrointestinal signs, the positivity rate for was 5 % (75/1496). Among the positive cases, 93.3 % were pediatric, with a mean age of 8.7 ± 7.7 years, and the highest prevalence (9 %) was observed in the 5-10 age group. The most commonly reported symptoms included abdominal pain (49.3 %), perianal itching (40 %), and nocturnal drooling (33.3 %). Molecular analysis revealed that all isolates belonged to genotype B, and eight haplotypes were identified. Six haplotypes were novel, while two haplotypes were identical to sequences previously reported from Iran, Iraq, and Greece. Although the prevalence of in our region was relatively low, it remains a significant public health concern, especially among school-aged children. Genotype B, widely reported in Europe and the Middle East, was dominant in our study population. Moreover, haplotypes in this study were identical or closely related to those from neighboring countries. Further studies from different regions of Turkey are needed for a more comprehensive epidemiological assessment.
是最常见的肠道蠕虫之一，尤其是在儿童中。线粒体1基因分析揭示了三种基因型（A、B、C）。我们旨在评估在梅尔辛大学医院诊断的病例的流行病学特征和1基因型。2017年1月至2023年12月期间，使用透明胶带法检查了1496份样本，确定了75份阳性样本。对流行病学数据进行了回顾性分析。通过酚氯仿法从30份阳性样本中分离DNA，并对1基因的巢式PCR产物进行测序。使用GenBank序列进行系统发育分析。在有胃肠道症状的患者样本中，的阳性率为5%（75/1496）。在阳性病例中，93.3%为儿科患者，平均年龄为8.7±7.7岁，5-10岁年龄组的患病率最高（9%）。最常报告的症状包括腹痛（49.3%）、肛周瘙痒（40%）和夜间流口水（33.3%）。分子分析显示，所有分离株均属于基因型B，并鉴定出8种单倍型。6种单倍型是新的，而2种单倍型与先前在伊朗、伊拉克和希腊报道的序列相同。尽管我们地区的患病率相对较低，但它仍然是一个重大的公共卫生问题，尤其是在学龄儿童中。在欧洲和中东广泛报道的基因型B在我们的研究人群中占主导地位。此外，本研究中的单倍型与邻国的单倍型相同或密切相关。需要来自土耳其不同地区的进一步研究进行更全面的流行病学评估。
