伊朗西北部地区分离株的基因型分布。
Genotype Distribution of Isolates from Northwest Provinces of Iran.
机构信息
Department of Biology, Urmia Branch, Islamic Azad University, Urmia, Iran.
Cellular and Molecular Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran.
出版信息
Ethiop J Health Sci. 2023 May;33(3):433-440. doi: 10.4314/ejhs.v33i3.6.
BACKGROUND
Human infection with occurs worldwide. The most common clinical manifestation of a pinworm infection is an itchy anal region. This parasite is incidentally found in appendicitis. This study aims to characterize and genotype this parasite from different samples inferred by mt-DNA.
METHODS
Forty appendectomies for acute clinical appendicitis, 40 positive scotch-tape samples, and 10 adult females worm isolated from patients. Genetic differentiation, haplotype differences, and isolates population structure were analyzed based on the cytochrome c oxidase subunit I (1) gene.
RESULTS
It has been demonstrated that all isolations in the appendectomies specimens are similar, and the genetic difference divergence is seen in adult worm specimens. The neutral indices of the samples did not show a significant difference and show that there is no intra-specific and population distribution diversity.
CONCLUSION
Our results show different haplotypes in the B type of E. vermicularis population and add new information about genotyping of these parasites in Iran. In comparison with other studies, intra-specific variation of this parasite from Iran was observed.
背景
在全球范围内都有人感染 的情况。蛲虫感染最常见的临床表现是肛门瘙痒。这种寄生虫在阑尾炎中偶然发现。本研究旨在通过 mt-DNA 推断不同样本中的这种寄生虫并对其进行基因分型。
方法
对 40 例急性临床阑尾炎的阑尾切除术、40 例阳性胶带样本和 10 例从患者中分离出的成年雌性蠕虫进行研究。基于细胞色素 c 氧化酶亚基 I (1) 基因分析遗传分化、单倍型差异和分离株种群结构。
结果
研究表明，阑尾切除术标本中的所有分离株都相似，而成年蠕虫标本中则存在遗传差异。样本的中性指标没有显示出显著差异，表明没有种内和种群分布多样性。
结论
我们的结果显示，在 B 型 种群中存在不同的单倍型，并为伊朗这些寄生虫的基因分型提供了新的信息。与其他研究相比，伊朗的这种寄生虫存在种内变异。
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