人胚肾（HEK）细胞系的非靶向代谢组学

Untargeted Metabolomics of Human Embryonic Kidney (HEK) Cell Line.

作者信息

Du Xing, Gomez Claudia, Li Susanna, Dorairaj Emily, Jain Samyak, Bhattacharya Sanjoy K

机构信息

Bascom Palmer Eye Institute, Miami Integrative Metabolomics Research Center, University of Miami, Miami, FL, USA.

Jinan Second People's Hospital, Jinan, Shandong, People's Republic of China.

出版信息

Methods Mol Biol. 2025;2925:297-308. doi: 10.1007/978-1-0716-4534-5_22.

DOI:10.1007/978-1-0716-4534-5_22

PMID:40498199

Abstract

Human embryonic kidney (HEK) cells' low maintenance and quick proliferation make it an ideal target to begin cellular research. Current literature on the metabolomic changes in the fatty acids synthesis (FAS) process is sparse. Thus, inhibition of the pathway in extensively researched HEK cells would provide further insight into upregulation and downregulation of metabolites. Liquid chromatography-mass spectrometry allows for highly sensitive quantification and qualification to provide a comprehensive understanding of the metabolomic changes that can be applied to specialized cell types.

摘要

人胚肾（HEK）细胞易于培养且增殖迅速，使其成为开展细胞研究的理想对象。目前关于脂肪酸合成（FAS）过程中代谢组学变化的文献较少。因此，在深入研究的HEK细胞中抑制该途径将有助于进一步了解代谢物的上调和下调情况。液相色谱 - 质谱联用技术能够进行高灵敏度的定量和定性分析，从而全面了解可应用于特定细胞类型的代谢组学变化。