Shen Yue, Lin Lu, Yu Weijun, Cui Zhurong, Lin Tingting, Hu Shucheng, Shi Yuanjie, Gu Yuting, Jin Min, Lu Eryi
Department of Stomatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Stomatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Int Dent J. 2025 Jun 10;75(4):100837. doi: 10.1016/j.identj.2025.100837.
Carnitine palmitoyltransferase I (CPT1), the rate-limiting enzyme of fatty acid oxidation, plays a crucial role in inflammation. However, the relation between CPT1 and periodontitis is obscure. This study aimed to reveal the expression pattern of CPT1 (CPT1A, CPT1B, CPT1C) in periodontitis and their correlation with clinical parameters.
The public dataset was used to identify biological processes related to lipid metabolism and validate the differential expression of CPT1 between healthy and periodontitis groups. Forty-seven healthy gingival samples and 47 periodontitis gingival samples were gathered. Subsequently, real-time quantitative polymerase chain reaction was used to measure CPT1 expression. Their protein levels were assessed using western blotting and histological analysis. Single-cell analysis using GSE164241 was conducted to observe CPT1A and CPT1C expression in different cell types, followed by immunofluorescence validation. Finally, the correlation of CPT1A and CPT1C with clinical parameters was estimated, and receiver operating characteristic curves were constructed.
Most biological processes related to lipid metabolism shared the gene CPT1A, and the expression of CPT1A and CPT1C in the periodontitis group was upregulated. Besides, both CPT1A and CPT1C were expressed mainly in endothelial cells, and their expression was elevated in endothelial cells of periodontitis. Furthermore, CPT1A and CPT1C were correlated with clinical parameters, and CPT1A could serve as a diagnostic marker of periodontitis.
Our findings elucidated the expression of CPT1 in gingival tissues of periodontitis and demonstrated the association of CPT1 with periodontitis.
This study found the positive correlation of the expression of CPT1A and CPT1C in gingival tissues with clinical parameters, which might lay a foundational framework for further elucidating the underlying mechanisms of lipid metabolism and periodontitis and pave the way for exploring potential therapeutic targets.
肉碱棕榈酰转移酶I（CPT1）是脂肪酸氧化的限速酶，在炎症中起关键作用。然而，CPT1与牙周炎之间的关系尚不清楚。本研究旨在揭示CPT1（CPT1A、CPT1B、CPT1C）在牙周炎中的表达模式及其与临床参数的相关性。
使用公共数据集来识别与脂质代谢相关的生物学过程，并验证健康组和牙周炎组之间CPT1的差异表达。收集了47份健康牙龈样本和47份牙周炎牙龈样本。随后，使用实时定量聚合酶链反应来测量CPT1的表达。使用蛋白质印迹法和组织学分析评估其蛋白质水平。使用GSE164241进行单细胞分析，以观察CPT1A和CPT1C在不同细胞类型中的表达，随后进行免疫荧光验证。最后，评估CPT1A和CPT1C与临床参数的相关性，并构建受试者工作特征曲线。
大多数与脂质代谢相关的生物学过程共享基因CPT1A，牙周炎组中CPT1A和CPT1C的表达上调。此外，CPT1A和CPT1C均主要在内皮细胞中表达，并且它们在牙周炎内皮细胞中的表达升高。此外，CPT1A和CPT1C与临床参数相关，并且CPT1A可作为牙周炎的诊断标志物。
我们的研究结果阐明了CPT1在牙周炎牙龈组织中的表达，并证明了CPT1与牙周炎的关联。
本研究发现牙龈组织中CPT1A和CPT1C的表达与临床参数呈正相关，这可能为进一步阐明脂质代谢和牙周炎的潜在机制奠定基础框架，并为探索潜在治疗靶点铺平道路。
