对从猪身上分离出的奇异变形杆菌中参与抗菌抗性的基因组岛的研究。
Investigation of genomic islands involved in antimicrobial resistance in Proteus mirabilis isolated from swine.
作者信息
Su Hui, Yang Lirong, Liu Mimi, Pan Xiaorui, Li Hannuo, Sha Wanli, Yin Baishuang, Dong Wenlong, Li Guojiang
机构信息
College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, China.
College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, China; Jilin Provincial Key Laboratory of Preventive Veterinary Medicine, Jilin Agricultural Science and Technology University, Jilin, China; Jilin Province Technology Innovation Center of Pig Ecological Breeding and Disease Prevention and Control, Jilin Agricultural Science and Technology University, Jilin, China.
出版信息
J Glob Antimicrob Resist. 2025 Jun 9;44:160-165. doi: 10.1016/j.jgar.2025.06.003.
OBJECTIVES
Proteus mirabilis (P. mirabilis), a Gram-negative, rod-shaped bacterium, is ubiquitously present in natural environments as well as within the intestinal tracts of humans and animals. We isolated P. mirabilis SH-4 from a swine sample and investigated its antibiotic resistance genes (ARGs) along with their genetic structure.
METHODS
In this study, we have performed antimicrobial susceptibility testing by the broth microdilution method and conducted a comprehensive analysis through whole genome sequencing (WGS) and phylogenomics of P. mirabilis strain SH-4.
RESULTS
P. mirabilis SH-4 was found to possess a single chromosome measuring 4 123 479 base pairs in length, with a GC content of 39.06%. Besides, P. mirabilis SH-4 exhibited resistance to eleven antimicrobial agents tested. Through single nucleotide polymorphism (SNP) profiling, we revealed the genetic relationship of SH-4 with other P. mirabilis strains carrying various ARGs. WGS analysis revealed that SH-4 harbored a ΔTn7 transposon and two distinct genomic islands (GIs), designated as PmGRI-9-SH-4 and PmGRI-10-SH-4. ΔTn7 was identified, corresponding to Tn7 with an insertion of IS256 within its sequence. PmGRI-9-SH-4 displayed partial homology to PmGRI1-STP3 and PmGRI1-YN9 (China:Sichuan). Additionally, PmGRI-10-SH-4 contained composite transposons and eleven Type IV secretion system (T4SS) related genes, including traN, traU, traC, and others, with the MDR region situated near the terminus of this GI.
CONCLUSIONS
In summary, GIs and ISs may serve as crucial facilitators in the acquisition of resistance genes in P. mirabilis. These genetic elements turn P. mirabilis into highly resistant bacteria, culminating in infections that pose significant therapeutic challenges.
目的
奇异变形杆菌（Proteus mirabilis，P. mirabilis）是一种革兰氏阴性杆状细菌，广泛存在于自然环境以及人类和动物的肠道中。我们从一份猪样本中分离出奇异变形杆菌SH-4，并研究了其抗生素抗性基因（ARGs）及其遗传结构。
方法
在本研究中，我们采用肉汤微量稀释法进行了药敏试验，并通过全基因组测序（WGS）和奇异变形杆菌SH-4菌株的系统发育基因组学进行了全面分析。
结果
发现奇异变形杆菌SH-4拥有一条长度为4 123 479个碱基对的单一染色体，GC含量为39.06%。此外，奇异变形杆菌SH-4对所测试的11种抗菌药物表现出抗性。通过单核苷酸多态性（SNP）分析，我们揭示了SH-4与其他携带各种ARGs的奇异变形杆菌菌株之间的遗传关系。WGS分析表明，SH-4含有一个ΔTn7转座子和两个不同的基因组岛（GIs），分别命名为PmGRI-9-SH-4和PmGRI-10-SH-4。鉴定出ΔTn7，其对应于Tn7，在其序列中插入了IS256。PmGRI-9-SH-4与PmGRI1-STP3和PmGRI1-YN9（中国：四川）显示出部分同源性。此外，PmGRI-10-SH-4包含复合转座子和11个IV型分泌系统（T4SS）相关基因，包括traN、traU、traC等，多药耐药区域位于该GI的末端附近。
结论
总之，基因组岛（GIs）和插入序列（ISs）可能是奇异变形杆菌获得抗性基因的关键促进因素。这些遗传元件使奇异变形杆菌成为高度耐药的细菌，最终导致感染，带来重大的治疗挑战。
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