Olutoyin Adewusi Titilayomi Olufunso, Isbilen Ovgu
Faculty of Pharmacy, Cyprus International University, Nicosia, Northern Cyprus, via Mersin, Turkey/Biotechnology Research Center, Cyprus International University, Nicosia, Northern Cyprus, via Mersin, Turkey.
Faculty of Pharmacy, Cyprus International University, Nicosia, Northern Cyprus, via Mersin, Turkey/Faculty of Art and Sciences, Cyprus International University, Nicosia, Northern Cyprus, via Mersin, Turkey/Biotechnology Research Center, Cyprus International University, Nicosia, Northern Cyprus, via Mersin, Turkey.
Pak J Pharm Sci. 2025 Mar-Apr;38(2):679-690.
Annona muricata belongs to the family Annonaceae and the leaf extract has traditionally been used as an antipyretic, anti-diabetic, antihypertensive, and antimalarial. The study aims to formulate zinc oxide nanoparticles (ZnO NPs) using Annona muricata leaf extract (AME) and evaluate its biological activities. The biosynthesized ZnO NPs were characterized using UV-vis spectroscopy, FTIR, XRD and SEM. GC-MS analysis of AME indicated sesquiterpenes, acetogenins, alkaloids, flavonoids, fatty acids and other compounds. The presence of ZnO NPs was confirmed by the UV-vis absorption spectrum with a peak at 370 nm. XRD and FTIR showed the stabilized ZnO NPs using AME bioactive compounds with an average particle size of 79.14 nm. SEM and EDX revealed spherical and hexagonal morphology. Minimum inhibitory concentration experiments revealed that MIC values for P. aeruginosa and B. subtilis were 1.25mg/mL and 0.32mg/mL, respectively. Antioxidant activities of the AME, AME-ZnO NPs and ascorbic acid (standard) using DPPH assay revealed IC values of 2.17mg/mL, 1.66mg/mL and 0.23mg/mL, respectively. The formulated ZnO NPs also aided in the photocatalysis of methylene blue at a degradation rate of 66.8% after 180 min. These results collectively highlight the promising therapeutic potential of A. muricata biogenic zinc oxide nanoparticles for developing future therapies due to antibacterial, photocatalytic and antioxidant activities.
刺果番荔枝属于番荔枝科，其叶提取物传统上被用作退烧药、抗糖尿病药、抗高血压药和抗疟疾药。本研究旨在利用刺果番荔枝叶提取物（AME）制备氧化锌纳米颗粒（ZnO NPs）并评估其生物活性。采用紫外可见光谱、傅里叶变换红外光谱、X射线衍射和扫描电子显微镜对生物合成的ZnO NPs进行了表征。AME的气相色谱-质谱分析表明含有倍半萜、产乙酸素、生物碱、黄酮类、脂肪酸和其他化合物。紫外可见吸收光谱在370nm处有一个峰值，证实了ZnO NPs的存在。X射线衍射和傅里叶变换红外光谱表明，AME生物活性化合物稳定了ZnO NPs，平均粒径为79.14nm。扫描电子显微镜和能谱分析显示其形态为球形和六边形。最低抑菌浓度实验表明，铜绿假单胞菌和枯草芽孢杆菌的MIC值分别为1.25mg/mL和0.32mg/mL。采用DPPH法测定AME、AME-ZnO NPs和抗坏血酸（标准品）的抗氧化活性，IC值分别为2.17mg/mL、1.66mg/mL和0.23mg/mL。制备的ZnO NPs还能促进亚甲基蓝的光催化作用，180分钟后降解率为66.8%。这些结果共同突出了刺果番荔枝生物源氧化锌纳米颗粒由于其抗菌、光催化和抗氧化活性在开发未来疗法方面具有广阔的治疗潜力。
