Steinmeyer S, Tiebach R, Weber R
Z Lebensm Unters Forsch. 1985 Sep;181(3):198-9. doi: 10.1007/BF02425577.
A method for the analysis of deoxynivalenol (DON) und nivalenol (NV) in cereals is described. This method is suitable for routine use for control purposes. The extract is purified on a florisil column. Derivatization with heptafluorobutyrylimidazole is followed by quantitative determination by means of capillary gas chromatography and electron capture detection (ECD). For verification purposes two-dimensional thin layer chromatography is applied to gc-positive extracts. 67 cereal samples and cereal products from the Berlin market place have been assayed. Depending on the type of sample the limit of detection was 20 to 30 micrograms/kg for DON, and 100 to 300 micrograms/kg for NV.
本文描述了一种分析谷物中脱氧雪腐镰刀菌烯醇（DON）和雪腐镰刀菌烯醇（NV）的方法。该方法适用于常规控制目的。提取物在弗罗里硅土柱上进行纯化。用七氟丁酰咪唑衍生化后，通过毛细管气相色谱和电子捕获检测（ECD）进行定量测定。为进行验证，对气相色谱阳性提取物应用二维薄层色谱法。已对来自柏林市场的67个谷物样品和谷物产品进行了分析。根据样品类型，DON的检测限为20至30微克/千克，NV的检测限为100至300微克/千克。
