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[中性脂质的死后分解。现代分析方法(高效液相色谱法、毛细管气相色谱法、气相色谱-质谱联用仪和核磁共振)在尸蜡形成中的应用]

[Postmortem decomposition of neutral lipids. Use of modern methods of analysis (HPLC, capillary GC, GC-MS and NMR) in adipocere formation].

作者信息

Szathmary S C, von Tamaska L, Steigel A

出版信息

Z Rechtsmed. 1985;94(4):273-87. doi: 10.1007/BF00205095.

Abstract

The chemical composition of subcutaneous fats was analyzed in a corpse that had died from drowning. The skin of the cadaver examined postmortem showed different stages of adipocere. Samples from these regions were chemically compared with the fatty tissue of a person who had died recently. HPLC, GC, GC-MS, NMR (1H- and 13C-NMR), TLC and titrimetrical methods were used to evaluate the degree of decomposition. The fatty acid pattern of the triglycerides (TG) and the free fatty acids (FFA) obtained by TLC separation was also investigated. Some discrepancy was observed between the autopsy findings and the results of the chemical analysis. It is suggested that the autopsy should be supplemented by chemical analysis in order to describe the state of adipocere correctly.

摘要

对一名溺水死亡尸体的皮下脂肪化学成分进行了分析。尸检时检查的尸体皮肤呈现出不同阶段的尸蜡化。将这些区域的样本与近期死亡者的脂肪组织进行化学比较。采用高效液相色谱法(HPLC)、气相色谱法(GC)、气相色谱 - 质谱联用法(GC-MS)、核磁共振法(1H-和13C-NMR)、薄层色谱法(TLC)和滴定法来评估分解程度。还研究了通过TLC分离得到的甘油三酯(TG)和游离脂肪酸(FFA)的脂肪酸模式。在尸检结果和化学分析结果之间观察到了一些差异。建议通过化学分析来补充尸检,以便正确描述尸蜡化状态。

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