Gao Hongxiao, Xu Jing, Wu Manyan, Yang Yuemeng, Li Na, Chen Hong, Xu Li-Ping
Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, P. R. China.
Suzhou Institute for Advanced Research University of Science and Technology of China, Suzhou 215123, P. R. China.
Anal Chem. 2025 Jun 24;97(24):12623-12632. doi: 10.1021/acs.analchem.5c00891. Epub 2025 Jun 12.
Acute myocardial infarction (AMI) poses a threat to human health, and current clinical diagnostic methods cannot achieve early warning of AMI. The accurate detection of AMI-specific miRNAs is of great significance for the early diagnosis and treatment of AMI. However, the low abundance of miRNAs poses a major challenge to the sensitive detection of ultratrace miRNAs. Herein, a superwettable SERS microchip with an evaporation-induced SERS enhancement effect was developed for AMI-specific miRNA detection, which integrated a superwettable patterned surface and DNA walker strategy. Benefiting from the extreme difference in wettability between the superhydrophilic microwell and superhydrophobic background, both target and SERS tag can be sufficiently enriched in the superhydrophilic microwell. Abundant hotspots are generated through a simple evaporation-induced concentration and aggregation, leading to highly responsive and reproducible SERS signals. Coupled with the DNA walker strategy for signal amplification, the prepared superwettable SERS microchip enables the sensitive and multiplexed detection of AMI-specific miRNAs in both buffer and whole serum without interference. Furthermore, the fabricated microchip demonstrates accurate quantification of AMI-specific miRNAs in clinical samples, enabling differentiation among AMI patients, non-AMI individuals presenting with chest pain, and healthy individuals. This advancement provides a facile approach for the accurate diagnosis and early warning of AMI. We envision that this work will open new avenues for the fabrication of SERS biosensors and hold promise for medical diagnostics.
急性心肌梗死（AMI）对人类健康构成威胁，而目前的临床诊断方法无法实现对AMI的早期预警。准确检测AMI特异性微小RNA（miRNA）对AMI的早期诊断和治疗具有重要意义。然而，miRNA的低丰度对超痕量miRNA的灵敏检测构成了重大挑战。在此，我们开发了一种具有蒸发诱导表面增强拉曼散射（SERS）效应的超润湿性SERS微芯片用于检测AMI特异性miRNA，该芯片集成了超润湿性图案化表面和DNA步行器策略。受益于超亲水微孔与超疏水背景之间的极端润湿性差异，靶标和SERS标签均可在超亲水微孔中充分富集。通过简单的蒸发诱导浓缩和聚集产生大量热点，从而产生高响应性和可重复性的SERS信号。结合用于信号放大的DNA步行器策略，所制备的超润湿性SERS微芯片能够在无干扰的情况下对缓冲液和全血清中的AMI特异性miRNA进行灵敏且多重的检测。此外，所制备的微芯片能够对临床样本中的AMI特异性miRNA进行准确定量，从而区分AMI患者、表现出胸痛症状的非AMI个体以及健康个体。这一进展为AMI的准确诊断和早期预警提供了一种简便方法。我们设想这项工作将为SERS生物传感器的制造开辟新途径，并有望用于医学诊断。
