Balem Joseph, Tan Chenjiao, Dias Nathália Cássia Ferreira, Arnold Mariah, Tran Sorrel, Severns Paul M, Teixeira Paulo J P L, Li Changying, Yang Li
Department of Plant Pathology, University of Georgia, Athens 30602, USA.
Department of Agricultural and Biological Engineering, University of Florida, Gainesville, FL, 32611, USA.
bioRxiv. 2025 May 28:2025.05.23.655667. doi: 10.1101/2025.05.23.655667.
Repairing a damaged body part is critical for the survival of any organism. In plants, tissue damage induces rapid responses that activate defense, regeneration and wound healing. While early wound signaling mediated by phytohormones, electrical signals and reactive oxygen species is well-characterized, the mechanisms governing the final stages of wound healing remain poorly understood. Here, we show that wounding in Arabidopsis leaves induces localized cooling, likely due to evaporative water loss, accompanied by the activation of cold-responsive genes. The subsequent disappearance of localized cooling and deactivation of cold-responsive genes serve as a quantitative marker of wound healing. Based on these observations, we developed a workflow by leveraging computer vision and deep learning to monitor the dynamics of wound healing. We found that CBFs transcription factors relay injury-induced cooling signal to wound healing. Thus, our work advances our understanding of tissue repair and provides a tool to quantify wound healing in plants.
修复受损身体部位对任何生物体的生存都至关重要。在植物中，组织损伤会引发快速反应，激活防御、再生和伤口愈合机制。虽然由植物激素、电信号和活性氧介导的早期伤口信号已得到充分研究，但伤口愈合最后阶段的调控机制仍知之甚少。在这里，我们表明拟南芥叶片受伤会引发局部降温，这可能是由于水分蒸发流失所致，同时伴随着冷响应基因的激活。随后局部降温的消失以及冷响应基因的失活是伤口愈合的定量标志。基于这些观察结果，我们利用计算机视觉和深度学习开发了一个工作流程来监测伤口愈合的动态过程。我们发现CBF转录因子将损伤诱导的降温信号传递至伤口愈合过程。因此，我们的工作增进了我们对组织修复的理解，并提供了一种量化植物伤口愈合的工具。
