Li Sijie, Fan Tianyi, Hao Zengyao, Zhang Zizhou, Han Xuetao, Li Jiayuan, Wang Yu, Zhou Huandi, Xue Xiaoying
Department of Radiotherapy, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, People's Republic of China.
Department of Central Laboratory, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, People's Republic of China.
Int J Gen Med. 2025 Jun 6;18:2923-2940. doi: 10.2147/IJGM.S521390. eCollection 2025.
The goal of this study was to analyze in depth the importance of the thymosin beta 4 X-linked gene (TMSB4X) in the disease process of gliomas for the prediction of patient prognosis.
We explored the expression of TMSB4X by analyzing datasets from The Cancer Genome Atlas (TCGA) datasets and the Chinese Glioma Genome Atlas (CGGA). Tumor sample tissues and corresponding paracancerous tissues of glioma patients were collected, and TMSB4X expression in glioma tissues was analyzed using Real-Time PCR to investigate its prognostic significance in glioma patients. In addition, we have plotted the ROC curves for the survival rate, performed univariate and multivariate Cox analyses and constructed nomogram. Differential expression analyses were performed on the basis of median expression levels of the TMSB4X gene, and Intuitive graphical presentation and visualisation of the 403 proteins screened were carried out using the Cytoscape software platform. (version: 3.9.1). The top 50 core genes were then screened using the cyto Hubba algorithm, these genes were analyzed using The Gene Ontology Database (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). TCGA datasets was utilized for Gene Set Enrichment Analysis (GSEA) to obtain potential NF-κB-related pathways in gliomas.
Independent prognostic analyses show that the TMSB4X gene was identified as an independent prognostic indicator for gliomas. In GOKEGG and GSEA analyses, the analyses indicate that TMSB4X may play a crucial role in glioma patients by up-regulating I-kappaB kinase/NF-kappaB signaling. In glioma patients, upregulation of the I-kappaB kinase and NF-kappaB signalling pathways plays a crucial role, which may be linked to CASP1.
The findings showed that TMSB4X was identified as an potential independent risk factor in the prognosis of glioma patients, a finding that implies that TMSB4X has the potential to serve as a key biomarker for predicting the prognostic status of glioma patients.
本研究的目的是深入分析胸腺素β4 X连锁基因（TMSB4X）在胶质瘤疾病进程中对预测患者预后的重要性。
我们通过分析来自癌症基因组图谱（TCGA）数据集和中国胶质瘤基因组图谱（CGGA）的数据集来探究TMSB4X的表达。收集胶质瘤患者的肿瘤样本组织和相应的癌旁组织，使用实时定量聚合酶链反应分析胶质瘤组织中TMSB4X的表达，以研究其在胶质瘤患者中的预后意义。此外，我们绘制了生存率的ROC曲线，进行了单因素和多因素Cox分析并构建了列线图。基于TMSB4X基因的中位表达水平进行差异表达分析，并使用Cytoscape软件平台（版本：3.9.1）对筛选出的403种蛋白质进行直观的图形展示和可视化。然后使用cyto Hubba算法筛选出前50个核心基因，使用基因本体数据库（GO）和京都基因与基因组百科全书（KEGG）对这些基因进行分析。利用TCGA数据集进行基因集富集分析（GSEA），以获得胶质瘤中潜在的NF-κB相关通路。
独立预后分析表明，TMSB4X基因被确定为胶质瘤的独立预后指标。在GO/KEGG和GSEA分析中，结果表明TMSB4X可能通过上调I-κB激酶/NF-κB信号通路在胶质瘤患者中发挥关键作用。在胶质瘤患者中，I-κB激酶和NF-κB信号通路的上调起着关键作用，这可能与半胱天冬酶-1（CASP1）有关。
研究结果表明，TMSB4X被确定为胶质瘤患者预后的潜在独立危险因素，这一发现意味着TMSB4X有潜力作为预测胶质瘤患者预后状态的关键生物标志物。
