Ike Masakazu, Yamagishi Kenji, Tokuyasu Ken
1 Research Center for Agricultural Robotics, NARO.
2 Food Research Institute, National Agriculture and Food Research Organization (NARO).
J Appl Glycosci (1999). 2025 May 20;72(2):7202203. doi: 10.5458/jag.7202203. eCollection 2025.
Here, we aimed to construct a biological saccharification process that combines the steps of enzyme production and enzymatic saccharification using an aerobic fungus , an excellent cellulase producer. Sugar production consists of the growth phase at 28 °C and the saccharification phase at 50 °C. Final sugar yields from alkali-treated rice straw and microcrystalline cellulose using the M2-1 strain were greatly affected by mycelial inoculum size and growth phase periods. The optimization of these factors yielded 74.5 % and 60.6 % of sugar from the alkali-treated rice straw and microcrystalline cellulose, respectively, at 120 h of the biological saccharification process. In comparison with the process employing anaerobic microorganisms, a relatively higher yield of sugars was achieved within a shorter period and the use of non-GM fungal strain. However, large variability in sugar yields based on feedstocks suggests imperceptible differences in initial conditions.
在此，我们旨在构建一个生物糖化过程，该过程结合了使用一种好氧真菌（一种出色的纤维素酶生产者）进行酶生产和酶促糖化的步骤。糖的生产包括在28°C的生长阶段和在50°C的糖化阶段。使用M2-1菌株从碱处理稻草和微晶纤维素中获得的最终糖产量受到菌丝接种量和生长阶段时间的极大影响。对这些因素进行优化后，在生物糖化过程120小时时，从碱处理稻草和微晶纤维素中分别获得了74.5%和60.6%的糖。与使用厌氧微生物的过程相比，在较短时间内实现了相对较高的糖产量，并且使用的是非转基因真菌菌株。然而，基于原料的糖产量存在很大差异，这表明初始条件存在细微差异。
