Ike Masakazu, Tokuyasu Ken
1 Food Biotechnology Division, Food Research Institute, National Agriculture and Food Research Organization (NARO).
J Appl Glycosci (1999). 2018 Nov 20;65(4):51-56. doi: 10.5458/jag.jag.JAG-2018_0005. eCollection 2018.
To expand the range of soluble carbon sources for our enzyme production system, we investigated the properties of sucrose utilization and its effect on cellulase production by M2-1. We performed batch cultivation of M2-1 on sucrose and related sugars along with cellobiose, which was used as a cellulase inducer. The results clearly revealed that the hydrolysis products of sucrose, glucose and fructose, but not sucrose, can be used as a carbon source for enzyme production. In a 10-day continuous feeding experiment using invertase-treated sucrose/cellobiose, the fungal strain produced cellulases with a filter paper-degrading activity of 20.3 U/mL and production efficiency of 254 U/g-carbon sources. These values were comparable with those of glucose/cellobiose feeding (21.2 U/mL and 265 U/g-carbon sources, respectively). Furthermore, the comparison of the specific activities clearly indicated that the compositions of both produced enzymes were similar. Therefore, enzymatically hydrolyzed sucrose can be utilized as an alternative carbon source to glucose in our enzyme production system with M2-1.
为了扩大我们酶生产系统中可溶性碳源的范围，我们研究了蔗糖利用特性及其对M2-1纤维素酶生产的影响。我们用蔗糖及相关糖类与用作纤维素酶诱导剂的纤维二糖对M2-1进行分批培养。结果清楚地表明，蔗糖的水解产物葡萄糖和果糖，而不是蔗糖，可作为酶生产的碳源。在使用转化酶处理的蔗糖/纤维二糖进行的为期10天的连续补料实验中，该真菌菌株产生的纤维素酶滤纸降解活性为20.3 U/mL，生产效率为254 U/g碳源。这些值与葡萄糖/纤维二糖补料（分别为21.2 U/mL和265 U/g碳源）的值相当。此外，比活性的比较清楚地表明，两种产生的酶的组成相似。因此，在我们用M2-1的酶生产系统中，酶解蔗糖可作为葡萄糖的替代碳源。
