Hall Richard, Bruce-Brand Cassandra, Mudini Washington, Aldera Alessandro Pietro
Department of Emergency Medicine, Wexford General Hospital, Wexford, Republic of Ireland.
Division of Anatomical Pathology, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.
Korean J Helicobacter Up Gastrointest Res. 2024 Mar;24(1):45-51. doi: 10.7704/kjhugr.2023.0048. Epub 2024 Mar 8.
The identification of is one of the main tasks of diagnostic histopathologists when evaluating endoscopic gastric biopsies. The sensitivity and specificity of different stains that facilitate this identification vary. Despite the existing guidelines, many histopathology laboratories perform routine histochemical staining of all gastric biopsies to improve turnaround times. This study assessed the utility of an immunohistochemical (IHC) stain compared with a routinely used histochemical stain, cresyl violet (CV), in the South African setting.
Cases were identified retrospectively, and original histopathology reports were used to establish the "ground truth" diagnoses. Three pathologists independently evaluated the CV and IHC stains; each pathologist was timed in a standardized manner. The sensitivity, specificity, interobserver variability, and time taken to identify with each stain were compared.
The overall sensitivity and specificity for IHC staining (85.2% and 97.7%, respectively) were higher than those for CV staining (64.5% and 90.6%, respectively). Detection of took an average of 16 and 49 seconds using the IHC and CV stains, respectively. The prevalence of in our laboratory was 23.7%, which is lower than the reported national prevalence in South Africa.
IHC stain-based detection of in inflamed gastric biopsies demonstrated superior sensitivity and specificity than CV staining. This was particularly true for cases involving patients with low bacterial loads. The interpretation of IHC staining is much faster than that associated with CV staining, which is important in centers with high caseloads and shortages of pathologists.
在内镜下胃活检评估中，识别[具体细菌名称未给出]是诊断组织病理学家的主要任务之一。有助于这种识别的不同染色方法的敏感性和特异性各不相同。尽管有现有指南，但许多组织病理学实验室对所有胃活检进行常规组织化学染色以缩短周转时间。本研究在南非环境中评估了一种[具体细菌名称未给出]免疫组织化学（IHC）染色与常规使用的组织化学染色甲酚紫（CV）相比的效用。
回顾性识别病例，并使用原始组织病理学报告确定“真实”诊断。三位病理学家独立评估CV和IHC染色；每位病理学家以标准化方式计时。比较了每种染色方法识别[具体细菌名称未给出]的敏感性、特异性、观察者间变异性和所需时间。
IHC染色的总体敏感性和特异性（分别为85.2%和97.7%）高于CV染色（分别为64.5%和90.6%）。使用IHC和CV染色检测[具体细菌名称未给出]平均分别需要16秒和49秒。我们实验室中[具体细菌名称未给出]的患病率为23.7%，低于南非报告的全国患病率。
基于IHC染色在炎症性胃活检中检测[具体细菌名称未给出]显示出比CV染色更高的敏感性和特异性。对于细菌载量低的患者病例尤其如此。[具体细菌名称未给出]IHC染色的解读比CV染色快得多，这在病例量大且病理学家短缺的中心很重要。
