Jenum P A
Acta Pathol Microbiol Immunol Scand C. 1985 Aug;93(4):175-82. doi: 10.1111/j.1699-0463.1985.tb02941.x.
An enzyme-linked immunosorbent assay (ELISA) for the detection of serum IgG and IgM antibodies against chlamydia group antigen is presented. 187 selected sera were tested both in the ELISA and in the complement-fixation test (CFT). Of 71 sera with negative results in the CFT, 19 (26.8%) were positive in the ELISA. Of 116 CFT positive sera, ten (8,6%) were ELISA negative. In 35 of 72 sera (48.6%) with CFT titre less than 40 chlamydial IgM was demonstrated, while 11 of 115 sera (9.5%) with CFT titre less than 40, were IgM positive. IgM may inhibit binding of IgG to the antigen by competition. The ELISA showed a greater discrimination between titre-values compared to the CFT. The results are presented as "estimated titers". This method is based on linear logarithmic dose-response curves. The method gives a continuous titre scale, is easily understood, is reproducible and may be used in seroepidemiology. One single serum dilution is required except for sera with high titre which have to be retested at a higher dilution.
本文介绍了一种用于检测血清中抗衣原体属抗原IgG和IgM抗体的酶联免疫吸附测定（ELISA）方法。选取了187份血清样本，同时采用ELISA法和补体结合试验（CFT）进行检测。在CFT检测结果为阴性的71份血清中，有19份（26.8%）在ELISA检测中呈阳性。在CFT检测结果为阳性的116份血清中，有10份（8.6%）在ELISA检测中呈阴性。在CFT滴度小于40的72份血清中，有35份（48.6%）检测出衣原体IgM，而在CFT滴度小于40的115份血清中，有11份（9.5%）IgM呈阳性。IgM可能通过竞争抑制IgG与抗原的结合。与CFT相比，ELISA在区分滴度值方面表现出更高的辨别力。结果以“估计滴度”表示。该方法基于线性对数剂量反应曲线。该方法提供了连续的滴度范围，易于理解，具有可重复性，可用于血清流行病学研究。除了高滴度血清需要以更高稀释度重新检测外，只需进行一次血清稀释。
