An enzyme-linked immunosorbent assay (ELISA) for the detection of chlamydial antibodies in caprine sera.

Chlamydia psittaci is one of the major cause of abortion in goats. The complement-fixation (CF) test is widely used for detection of chlamydial antibodies in caprine sera. A simple, rapid enzyme-linked immunosorbent assay (ELISA) has been developed for the measurement of immunoglobulins G (IgG). The Chlamydia psittaci strain AB3 grown in yolk sac was used as antigen. ELISA proved satisfactory for the detection of serum IgG; a total of 204 sera were tested and the results correlated well with those obtained by CF test (80 per cent of agreement). The results obtained with ELISA were reproductible.