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一种用于检测山羊血清中衣原体抗体的酶联免疫吸附测定(ELISA)。

An enzyme-linked immunosorbent assay (ELISA) for the detection of chlamydial antibodies in caprine sera.

作者信息

Pépin M, Bailly L, Souriau A, Rodolakis A

出版信息

Ann Rech Vet. 1985;16(4):393-8.

PMID:4091495
Abstract

Chlamydia psittaci is one of the major cause of abortion in goats. The complement-fixation (CF) test is widely used for detection of chlamydial antibodies in caprine sera. A simple, rapid enzyme-linked immunosorbent assay (ELISA) has been developed for the measurement of immunoglobulins G (IgG). The Chlamydia psittaci strain AB3 grown in yolk sac was used as antigen. ELISA proved satisfactory for the detection of serum IgG; a total of 204 sera were tested and the results correlated well with those obtained by CF test (80 per cent of agreement). The results obtained with ELISA were reproductible.

摘要

鹦鹉热衣原体是山羊流产的主要病因之一。补体结合(CF)试验广泛用于检测山羊血清中的衣原体抗体。已开发出一种简单、快速的酶联免疫吸附测定(ELISA)法来检测免疫球蛋白G(IgG)。用在卵黄囊中培养的鹦鹉热衣原体菌株AB3作为抗原。ELISA法在检测血清IgG方面效果良好;共检测了204份血清,结果与CF试验结果相关性良好(一致率达80%)。ELISA法所得结果具有可重复性。

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