Liao Haiping, Zhao Yunyi, Liang Yongheng, Zou Kang
College of Life Sciences, Key Laboratory of Agricultural Environmental Microbiology of Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, Nanjing 210095, China.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Nutrients. 2025 May 26;17(11):1800. doi: 10.3390/nu17111800.
Diabetic nephropathy (DN) represents a severe microvascular complication of diabetes mellitus with limited therapeutic options, many of which are accompanied by considerable adverse effects. (OFI) fruit, rich in vitamins, dietary fiber, and fatty acids, contains numerous bioactive compounds, including phytosterols, polysaccharides, and flavonoids that demonstrate significant potential in diabetes management. The flavonoid fraction derived from OFI fruit (OFI-F) has exhibited pronounced anti-inflammatory, antioxidant, and gut microbiota modulatory properties. However, the efficacy of OFI-F in ameliorating DN and its underlying mechanisms remain inadequately elucidated. This investigation examined the therapeutic potential of OFI-F in DN and explored its mechanistic pathways. Our findings demonstrate that OFI-F administration significantly attenuated renal injury and intestinal barrier dysfunction in the DN murine model. OFI-F intervention resulted in multiple beneficial outcomes in DN mice, including the mitigation of weight loss, reduction in hyperglycemia, decrease in renal coefficient index, and the attenuation of renal injury. An analysis of gut microbiota composition revealed that OFI-F administration favorably modulated the intestinal microbial community by enhancing the abundance of beneficial bacteria while concomitantly reducing populations of potentially pathogenic bacteria. Additionally, OFI-F treatment promoted the production of short-chain fatty acids (SCFAs), which contributed substantially to renoprotection and inflammatory resolution. Antibiotic intervention studies further confirmed the indispensable role of gut microbiota in mediating the renoprotective effects of OFI-F. In conclusion, this study provides compelling evidence supporting the therapeutic potential of OFI-F in DN management through the concurrent modulation of gut microbiota and renal function, offering a promising nutraceutical approach for alleviating renal injury in DN.
糖尿病肾病（DN）是糖尿病严重的微血管并发症，治疗选择有限，其中许多治疗方法都伴有相当大的不良反应。富含维生素、膳食纤维和脂肪酸的油橄榄果实（OFI）含有多种生物活性化合物，包括植物甾醇、多糖和黄酮类化合物，这些化合物在糖尿病管理中显示出巨大潜力。从油橄榄果实中提取的黄酮类成分（OFI-F）具有显著的抗炎、抗氧化和调节肠道微生物群的特性。然而，OFI-F改善DN的疗效及其潜在机制仍未得到充分阐明。本研究考察了OFI-F在DN中的治疗潜力，并探索了其作用机制。我们的研究结果表明，给予OFI-F可显著减轻DN小鼠模型的肾损伤和肠道屏障功能障碍。OFI-F干预在DN小鼠中产生了多种有益结果，包括减轻体重减轻、降低高血糖、降低肾系数指数以及减轻肾损伤。对肠道微生物群组成的分析表明，给予OFI-F可通过增加有益菌的丰度，同时减少潜在致病菌的数量，从而有利地调节肠道微生物群落。此外，OFI-F治疗促进了短链脂肪酸（SCFAs）的产生，这对肾脏保护和炎症消退有很大贡献。抗生素干预研究进一步证实了肠道微生物群在介导OFI-F肾脏保护作用中的不可或缺的作用。总之，本研究提供了有力证据，支持OFI-F通过同时调节肠道微生物群和肾功能来管理DN的治疗潜力，为减轻DN肾损伤提供了一种有前景的营养补充方法。
