Luo Jichu, Wei Zhijuan, Tan Yuru, Tong Ying, Yang Bao, Wen Mingsen, Guan Xuan, Zhu Pingchuan, Xu Song, Lin Xueting, Zhang Qisong
Guangxi Key Laboratory of Special Biomedicine, School of Medicine, Guangxi University, Nanning 530004, China.
Hubei Provincial Key Laboratory of Occurrence and Intervention of Rheumatic Diseases, Hubei Minzu University, Enshi 445000, China.
Nutrients. 2025 Aug 18;17(16):2665. doi: 10.3390/nu17162665.
: Type 2 diabetes (T2D) has become a serious global public health concern. Liubao tea (LBT) has demonstrated beneficial effects on gut microbiota and glucose-lipid metabolism, holding promising therapeutic potential for T2D; however, its underlying mechanisms remain unclear. This study aims to elucidate the potential mechanisms of Liubao tea extract (LBTE) against T2D. : LC-MS technology was used to identify the chemical components of LBTE and combined with network pharmacology and molecular docking to screen its potential active ingredients and targets for improving T2D. Therapeutic efficacy was assessed in high-fat diet/streptozotocin (HFD/STZ)-induced diabetic mice via serum biochemical analyses and histopathological examinations. Serum metabolomics, 16S rRNA sequencing, quantification of short-chain fatty acids (SCFAs), quantitative real-time PCR (qPCR), and antibiotic-treated pseudo-germ-free models were employed to elucidate the underlying mechanisms. : LBTE effectively reduced blood glucose levels and improved lipid metabolism, primarily by promoting hepatic glycogen synthesis and suppressing glycerophospholipid synthesis. LBTE also alleviated hepatic inflammation by modulating inflammatory cytokine expression. Additionally, LBTE reshaped the gut microbiota profiles by decreasing harmful bacteria and increasing SCFA-producing bacteria, resulting in elevated fecal SCFAs. SCFAs contributed to improving hepatic metabolism and inflammation, enhancing intestinal barrier function. Notably, these effects were abolished by antibiotic-induced microbiota depletion, confirming the microbiota-dependent mechanism of LBTE. Quercetin, luteolin, genistein, and kaempferol were considered as potential active ingredients contributing to the antidiabetic effects of LBTE. : These findings provide novel perspectives on the viability of LBTE as a complementary strategy for T2D prevention and management.
2型糖尿病（T2D）已成为全球严重的公共卫生问题。六堡茶（LBT）已显示出对肠道微生物群和糖脂代谢的有益作用，对T2D具有潜在的治疗潜力；然而，其潜在机制仍不清楚。本研究旨在阐明六堡茶提取物（LBTE）抗T2D的潜在机制。采用液相色谱-质谱联用（LC-MS）技术鉴定LBTE的化学成分，并结合网络药理学和分子对接技术筛选其改善T2D的潜在活性成分和靶点。通过血清生化分析和组织病理学检查评估高脂饮食/链脲佐菌素（HFD/STZ）诱导的糖尿病小鼠的治疗效果。采用血清代谢组学、16S rRNA测序、短链脂肪酸（SCFAs）定量、定量实时聚合酶链反应（qPCR）和抗生素处理的伪无菌模型来阐明潜在机制。LBTE主要通过促进肝糖原合成和抑制甘油磷脂合成有效降低血糖水平并改善脂质代谢。LBTE还通过调节炎症细胞因子表达减轻肝脏炎症。此外，LBTE通过减少有害细菌和增加产生SCFA的细菌重塑肠道微生物群谱，导致粪便SCFAs升高。SCFAs有助于改善肝脏代谢和炎症，增强肠道屏障功能。值得注意的是，抗生素诱导的微生物群耗竭消除了这些作用，证实了LBTE的微生物群依赖性机制。槲皮素、木犀草素、染料木黄酮和山奈酚被认为是有助于LBTE抗糖尿病作用的潜在活性成分。这些发现为LBTE作为T2D预防和管理的补充策略的可行性提供了新的视角。
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