非乳糜泻性小麦麸质敏感模型：对Wistar大鼠肝脏形态生理学的影响

Non-Celiac Wheat Gluten Sensitivity Model: Effects on Hepatic Morphophysiology of Wistar Rats.

作者信息

Duarte Ana Luiza Russo, Silva Gabriela Barone Volce da, Santa Rosa Anne Caroline, Raniero Ghiovani Zanzotti, Monteiro Antonio Roberto Giriboni, de Souza Gustavo Henrique, Sá-Nakanishi Anacharis Babeto de, Comar Jurandir Fernando, Cuman Roberto Kenji Nakamura, Natali Maria Raquel Marçal

机构信息

Graduate Program in Biological Sciences, State University of Maringa, Maringa 87020-900, Brazil.

Department of Food Engineering, State University of Maringa, Maringa 87020-900, Brazil.

出版信息

Nutrients. 2025 May 28;17(11):1842. doi: 10.3390/nu17111842.

DOI:10.3390/nu17111842

PMID:40507110

Abstract

BACKGROUND/OBJECTIVES: Wheat gluten intolerance increases intestinal permeability, triggering inflammation that may directly affect liver function and compromise metabolic health.

METHODS

Male Wistar rats (n = 50) aged 21 days were divided into five groups (n = 10) based on dietary gluten levels over 100 days: G0 (0%), G14 (14%), G42 (42%), G70 (70%), and G70/0 (70% for the first 70 days, then 0% until euthanasia). At 121 days, the animals were weighed and euthanized, and blood samples were collected for biochemical analyses. Adipose tissue deposits and the liver were excised and weighed. Liver lobes were isolated and fixed for morphological and morphometric analysis of hepatocytes, tissue glycogen percentage, and intracellular lipid assessment.

RESULTS

The hepatic oxidative status was evaluated. The ingestion of diets with excess gluten (70%) increased final body mass and reduced liver mass, though it did not alter the adiposity index. Cholesterol, triglycerides, and myeloperoxidase enzyme activity exhibited distinct patterns across all groups.

CONCLUSIONS

Elevated gluten levels increased oxidative stress and altered tissue hepatic morphology and morphometry.

摘要

背景/目的：小麦麸质不耐受会增加肠道通透性，引发炎症，可能直接影响肝功能并损害代谢健康。

方法

将21日龄的雄性Wistar大鼠（n = 50）根据100天内的饮食麸质水平分为五组（n = 10）：G0（0%）、G14（14%）、G42（42%）、G70（70%）和G70/0（前70天为70%，然后直至安乐死为0%）。在121天时，对动物进行称重并安乐死，采集血样进行生化分析。切除脂肪组织沉积物和肝脏并称重。分离肝叶并固定，用于对肝细胞进行形态学和形态计量学分析、评估组织糖原百分比和细胞内脂质。