Choi Hye Ji, Lee Hyo Lim, Kim In Young, Heo Ho Jin
Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea.
Int J Mol Sci. 2025 May 25;26(11):5080. doi: 10.3390/ijms26115080.
Industrial development has improved living standards; however, mortality associated with fine particulate matter (PM) exposure continues to rise. Despite increasing awareness of its health risks, effective strategies to mitigate PM-induced pulmonary damage remain limited. This study examines the protective properties of an ethanolic extract from (EPM) in preventing pulmonary dysfunction induced by PM, as well as its possible use as a dietary intervention to improve respiratory health. The physiological compounds in EPM were identified using ultra-performance liquid chromatography, and its protective effects were evaluated via in vitro assays using A549 and RPMI 2650 cells. The antioxidant system and mitochondrial function were further analyzed in the lung tissues of PM-exposed BALB/c mice, with molecular mechanisms elucidated by Western blot analysis. The main bioactive compounds identified in EPM included 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside. EPM modulated the Nrf2 signaling pathway, enhancing antioxidant defense by regulating the expression of antioxidant-related proteins. Furthermore, EPM exhibited protective effects against inflammation, apoptosis, and fibrosis through the TLR4/p-JNK and TGF-β1 signaling pathways. These findings suggest that EPM exerts protective effects against PM-induced oxidative stress and inflammation and may be used as a functional food ingredient for respiratory health.
工业发展提高了生活水平；然而，与细颗粒物（PM）暴露相关的死亡率仍在上升。尽管人们对其健康风险的认识不断提高，但减轻PM引起的肺损伤的有效策略仍然有限。本研究考察了[植物名称]乙醇提取物（EPM）在预防PM诱导的肺功能障碍方面的保护特性，以及其作为改善呼吸健康的饮食干预措施的潜在用途。使用超高效液相色谱法鉴定了EPM中的生理化合物，并通过使用A549和RPMI 2650细胞的体外试验评估了其保护作用。对暴露于PM的BALB/c小鼠肺组织中的抗氧化系统和线粒体功能进行了进一步分析，并通过蛋白质免疫印迹分析阐明了分子机制。在EPM中鉴定出的主要生物活性化合物包括2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷。EPM调节Nrf2信号通路，通过调节抗氧化相关蛋白的表达增强抗氧化防御。此外，EPM通过TLR4/p-JNK和TGF-β1信号通路对炎症、细胞凋亡和纤维化表现出保护作用。这些发现表明，EPM对PM诱导的氧化应激和炎症具有保护作用，可作为改善呼吸健康的功能性食品成分。
