Chen Mingjie, Du Zhenghua, Yue Wenjie, Kong Xiangrui, Xu Quanming, Fang Dongsheng, Chen Changsong
College of Life Sciences, Xinyang Normal University, Xinyang 464000, China.
Tea Research Institute, Fujian Academy of Agricultural Sciences, The Fujian Research Branch of the National Center for Tea Genetic Improvement, Fuzhou 350012, China.
Int J Mol Sci. 2025 May 26;26(11):5094. doi: 10.3390/ijms26115094.
Bud paradormancy has been widely studied in perennial deciduous woody species, but little attention has been paid to paradormancy set and release in perennial evergreen tree species. Here, shoot bud paradormancy in cv. Huangdan was studied by untargeted metabolomics. We found that after removing the axillary floral buds for one day, the paradormancy of the axillary shoot buds was released. The paradormant shoot buds had lower glucose-1-phosphate, fructose, and D-(-)-tagatofuranose content but higher trehalose, raffinose, galactinol, and α-D-xylopyranose content. Meanwhile, high levels of asparagine were accumulated. Flavonoids were differentially accumulated, and higher levels of three flavone glycosides (C-diglucosylapigenin, apigenin 6-C-glucoside 8-C-arabinoside, and prunin) and four proanthocyanidins (Procyanidin trimer isomer 1, Galloylprocyanidin dimer, Procyanidin trimer isomer 3, and Galloylated trimeric proanthocyanidin) were accumulated in paradormant shoot buds. During the paradormancy-to-growth transition, all these metabolites were reversed. These data suggest that the reconfiguration of carbon, nitrogen, and flavonoid metabolism could be an important aspect for the paradormancy set and release of tea axillary shoot buds. This study provided novel insights into shoot bud paradormancy set and release in a perennial evergreen tree species.
芽的休眠在多年生落叶木本植物中已得到广泛研究，但多年生常绿树中芽休眠的建立与解除却很少受到关注。在此，通过非靶向代谢组学研究了黄旦品种的梢芽休眠。我们发现去除腋花芽一天后，腋生梢芽的休眠被解除。处于休眠状态的梢芽中葡萄糖-1-磷酸、果糖和D-(-)-塔格呋喃糖含量较低，但海藻糖、棉子糖、肌醇半乳糖苷和α-D-吡喃木糖含量较高。同时，积累了高水平的天冬酰胺。类黄酮差异积累，处于休眠状态的梢芽中三种黄酮苷（C-二葡萄糖基芹菜素、芹菜素6-C-葡萄糖苷8-C-阿拉伯糖苷和樱草糖苷）和四种原花青素（原花青素三聚体异构体1、没食子酰原花青素二聚体、原花青素三聚体异构体3和没食子酰化三聚体原花青素）积累水平较高。在从休眠到生长的转变过程中，所有这些代谢物的变化趋势相反。这些数据表明，碳、氮和类黄酮代谢的重新配置可能是茶树腋生梢芽休眠建立与解除的一个重要方面。本研究为多年生常绿树物种梢芽休眠的建立与解除提供了新的见解。
