Melnikova Daria N, Potapov Andrey E, Ovchinnikova Tatiana V, Bogdanov Ivan V
M.M. Shemyakin & Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, The Russian Academy of Sciences, 117997 Moscow, Russia.
Moscow Center for Advanced Studies, 123592 Moscow, Russia.
Int J Mol Sci. 2025 May 28;26(11):5169. doi: 10.3390/ijms26115169.
Pollen allergy is rated as a major public health problem, causing significant morbidity and adversely affecting the quality of people's lives. The airway epithelium serves as the first line of defense in the respiratory system, playing a crucial role in orchestrating immune responses to allergens. In this work, we studied the important transport steps in the major alder pollen allergen Aln g 1 through the human airway epithelium in comparison with those of the birch pollen allergen Bet v 1. Using fluorescence spectroscopy, we showed that both allergens can destroy liposomes with a composition modeling the adult human pulmonary surfactant. Using a polarized Calu-3 monolayer, we showed similar efficiencies of Aln g 1 and Bet v 1 transport through the artificial epithelial barrier. Using qPCR, we showed that Aln g 1 upregulates the expression of IL-33, TSLP, IL-1β, CXCL8 in epithelial cells, playing an important role in the sensitization process. The obtained results may improve our understanding of the primary sensitization mechanisms with the involvement of the PR-10 family of lipid-binding allergens.
花粉过敏被视为一个重大的公共卫生问题，会导致严重的发病率，并对人们的生活质量产生不利影响。气道上皮是呼吸系统的第一道防线，在协调对过敏原的免疫反应中起着关键作用。在这项工作中，我们研究了主要桤木花粉过敏原Aln g 1与桦树花粉过敏原Bet v 1相比，通过人气道上皮的重要转运步骤。利用荧光光谱法，我们表明这两种过敏原都能破坏具有模拟成人肺表面活性剂成分的脂质体。利用极化的Calu-3单层细胞，我们表明Aln g 1和Bet v 1通过人工上皮屏障的转运效率相似。利用qPCR，我们表明Aln g 1上调上皮细胞中IL-33、TSLP、IL-1β、CXCL8的表达，在致敏过程中起重要作用。所获得的结果可能会增进我们对涉及脂质结合过敏原PR-10家族的原发性致敏机制的理解。
