Huang Jieyi, Wei Xuan, Tang Min, Deng Ziqiu, Lan Yi, Liu Fang
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, Guangxi University, Nanning 530004, China.
Int J Mol Sci. 2025 May 30;26(11):5264. doi: 10.3390/ijms26115264.
Bacterial leaf streak (BLS) is one of the internationally significant quarantine diseases in rice. Effectively utilizing BLS resistance genes from wild rice ( Griff.) to breed new varieties offers a fundamental solution for BLS control. This study focused on the fine mapping of the BLS resistance gene and the development of closely linked molecular markers for breeding BLS-resistant lines. Using a Guangxi common wild rice accession DY19 (carrying ) as the donor parent and the highly BLS-susceptible indica rice variety 9311 as the recipient parent, BLS-resistant rice lines were developed through multiple generations of backcrossing and selfing, incorporating molecular marker-assisted selection (MAS), single nucleotide polymorphism(SNP) chip genotyping, pathogen inoculation assays, and agronomic trait evaluation. The results showed that was delimited to a 113 kb interval between the molecular markers ID2 and ID5 on chromosome 2, with both markers exhibiting over 98% accuracy in detecting . Four stable new lines carrying the segment were obtained in the BCF generation. These four lines showed highly significant differences in BLS resistance compared with 9311, demonstrating moderate resistance or higher with average lesion lengths ranging from 0.69 to 1.26 cm. Importantly, no significant differences were observed between these resistant lines and 9311 in key agronomic traits, including plant height, number of effective panicles, panicle length, seed setting rate, grain length, grain width, length-to-width ratio, and 1000-grain weight. Collectively, two molecular markers closely linked to were developed, which can be effectively applied in MAS, and four new lines with significantly enhanced resistance to BLS and excellent agronomic traits were obtained. These findings provide technical support and core germplasm resources for BLS resistance breeding.
细菌性条斑病（BLS）是水稻上具有国际重要性的检疫性病害之一。有效利用野生稻（Griff.）中的BLS抗性基因来培育新品种，为防治BLS提供了根本解决方案。本研究聚焦于BLS抗性基因的精细定位以及开发与之紧密连锁的分子标记，用于培育抗BLS品系。以广西普通野生稻DY19（携带 ）为供体亲本，高感BLS的籼稻品种9311为受体亲本，通过多代回交和自交，结合分子标记辅助选择（MAS）、单核苷酸多态性（SNP）芯片基因分型、病原菌接种试验和农艺性状评价，培育出抗BLS水稻品系。结果表明， 被定位到第2染色体上分子标记ID2和ID5之间113 kb的区间内，这两个标记在检测 时的准确率均超过98%。在回交一代（BCF）中获得了4个携带 片段的稳定新品系。这4个品系与9311相比，在BLS抗性上表现出极显著差异，表现为中抗或更高抗性，平均病斑长度在0.69至1.26 cm之间。重要的是，这些抗性品系与9311在株高、有效穗数、穗长、结实率、粒长、粒宽、长宽比和千粒重等关键农艺性状上未观察到显著差异。总体而言，开发了两个与 紧密连锁的分子标记，可有效应用于MAS，并获得了4个对BLS抗性显著增强且农艺性状优良的新品系。这些研究结果为BLS抗性育种提供了技术支持和核心种质资源。
