地佐环平不会改变大鼠和细胞培养物中的基因表达。

Dizocilpine Does Not Alter Gene Expression in Rats and in Cell Cultures.

作者信息

Matiiv Anton B, Rogoza Tatyana M, Razgovorova Irina A, Zhdanova Maria I, Trubitsina Nina P, Bezgina Mariya D, Isaeva Irina G, Markov Alexander G, Zhouravleva Galina A, Bondarev Stanislav A

机构信息

Department of Genetics and Biotechnology, St. Petersburg State University, 7/9 Universitetskaya emb., St. Petersburg 199034, Russia.

St. Petersburg Branch of the N.I. Vavilov Institute of General Genetics, St. Petersburg 199034, Russia.

出版信息

Int J Mol Sci. 2025 Jun 1;26(11):5329. doi: 10.3390/ijms26115329.

DOI:10.3390/ijms26115329

PMID:40508138

Abstract

The gene encodes the nitric oxide synthase 1 adaptor protein (NOS1AP), which binds to neuronal nitric oxide synthase (nNOS) and regulates nitric oxide (NO) production by dissociating nNOS from NMDA receptors (NMDARs). Notably, NOS1AP expression is upregulated upon NMDAR activation; however, there is no available data regarding its production under the receptor inhibition. The gene is also 1 among more than 1000 genes that are presumed to be associated with the development of schizophrenia. Various animal models of this disorder have been developed, some of which are based on the use of NMDAR antagonists such as dizocilpine (MK-801). In this study, we investigated the expression and production of NOS1AP in rats injected with a low dose of dizocilpine (0.1 mg/kg), as well as in SH-SY5Y and HEK293T cell lines treated with varying concentrations of the same compound (10-200 µM). According to our results, neither the expression of the gene nor the production of NOS1AP protein was affected by dizocilpine treatment.

摘要

该基因编码一氧化氮合酶1衔接蛋白（NOS1AP），它与神经元型一氧化氮合酶（nNOS）结合，并通过使nNOS与N-甲基-D-天冬氨酸受体（NMDARs）解离来调节一氧化氮（NO）的产生。值得注意的是，NMDAR激活后NOS1AP表达上调；然而，关于其在受体抑制情况下的产生情况尚无可用数据。该基因也是推测与精神分裂症发生相关的1000多个基因之一。已经开发了多种该疾病的动物模型，其中一些基于使用NMDAR拮抗剂，如地卓西平（MK-801）。在本研究中，我们研究了注射低剂量地卓西平（0.1 mg/kg）的大鼠以及用不同浓度的同一化合物（10 - 200 μM）处理的SH-SY5Y和HEK293T细胞系中NOS1AP的表达和产生情况。根据我们的结果，地卓西平处理既不影响该基因的表达，也不影响NOS1AP蛋白的产生。