Qin Mengfan, Liu Xiang, Song Jia, Zhao Feixue, Shi Yiji, Xu Yu, Guo Zhiting, Zhang Tianye, Wu Jiapeng, Wang Jinxiong, Li Wu, Li Keqi, Li Shimeng, Huang Zhen, Xu Aixia
College of Agronomy, Northwest A&F University, Yangling 712100, China.
Crop Research Institute, Guangdong Academy of Agricultural Science, Guangzhou 510640, China.
Plants (Basel). 2025 Jun 5;14(11):1719. doi: 10.3390/plants14111719.
The leaf plastochron serves as an indicator of the rate of leaf appearance, biomass accumulation, and branch number, while also impacting plant architecture and seed yield. However, research on the leaf plastochron of crops remains limited. In this study, 2116C exhibited a rapid leaf plastochron compared to ZH18 during both rosette and bud periods. There were significant positive correlations among the leaf plastochron and primary branch number of the F populations ( ranging from 0.395 to 0.635, < 0.01). Genetic analyses over two years demonstrated that two equally dominant genes might govern the leaf plastochron. Through bulk segregant analysis sequencing (BSA-seq), three novel genomic intervals were identified on chromosomes A02 (9.04-9.48 Mb and 13.52-13.66 Mb) and A04 (19.84-20.14 Mb) of ZS11 and Darmor- reference genomes. By gene functional annotations, single-nucleotide variation (SNV) analyses, transcriptome data from parents, genetic progeny, and natural accessions, we identified ten candidate genes within the intervals, including , , , , , , , and three genes. These findings lay the molecular foundation for further exploration into the leaf plastochron and the implications in plastochron-related breeding in rapeseed.
叶龄期是叶片出现速率、生物量积累和分枝数的指标，同时也影响植株形态和种子产量。然而，关于作物叶龄期的研究仍然有限。在本研究中，与ZH18相比，2116C在莲座期和现蕾期均表现出较快的叶龄期。F群体中叶龄期与一次分枝数之间存在显著正相关（范围为0.395至0.635，P<0.01）。两年的遗传分析表明，可能有两个等显性基因控制叶龄期。通过混合分组分析法测序（BSA-seq），在ZS11和Darmor-参考基因组的A02染色体（9.04-9.48 Mb和13.52-13.66 Mb）和A04染色体（19.84-20.14 Mb）上鉴定出三个新的基因组区间。通过基因功能注释、单核苷酸变异（SNV）分析、亲本、遗传后代和自然材料的转录组数据，我们在这些区间内鉴定出10个候选基因，包括[具体基因名称缺失]、[具体基因名称缺失]、[具体基因名称缺失]、[具体基因名称缺失]、[具体基因名称缺失]、[具体基因名称缺失]、[具体基因名称缺失]以及三个[具体基因类型缺失]基因。这些发现为进一步探索油菜叶龄期及其在叶龄期相关育种中的意义奠定了分子基础。
