Jiang Jiayi, Li Rihui, Wang Kaixuan, Xu Yifeng, Lu Hejun, Zhang Dongqing
Xianghu Laboratory, Hangzhou 311231, China.
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Int J Mol Sci. 2025 Jan 28;26(3):1148. doi: 10.3390/ijms26031148.
Cold tolerance in rapeseed is closely related to its growth, yield, and geographical distribution. However, the mechanisms underlying cold resistance in rapeseed remain unclear. This study aimed to explore cold resistance genes and provide new insights into the molecular mechanisms of cold resistance in rapeseed. Rapeseed (cold-sensitive line) and (cold-tolerant line) were used as parental lines. In their F population, 30 seedlings with the lowest cold damage levels and 30 with the highest cold damage levels were selected to construct cold-tolerant and cold-sensitive pools, respectively. The two pools and parental lines were analyzed using bulk segregant sequencing (BSA-seq). The G'-value analysis indicated a single peak on Chromosome C09 as the candidate interval, which had a 2.59 Mb segment with 69 candidate genes. Combined time-course and weighted gene co-expression network analyses were performed at seven time points to reveal the genetic basis of the two-parent response to low temperatures. Twelve differentially expressed genes primarily involved in plant cold resistance were identified. Combined BSA-seq and transcriptome analysis revealed , , and as the candidate genes. Quantitative real-time PCR validation of the candidate genes was consistent with RNA-seq. This study facilitates the exploration of cold tolerance mechanisms in rapeseed.
油菜的耐寒性与其生长、产量及地理分布密切相关。然而，油菜抗寒的潜在机制仍不清楚。本研究旨在探索抗寒基因，并为油菜抗寒的分子机制提供新的见解。以油菜（冷敏感系）和（耐寒系）作为亲本系。在其F群体中，分别选择30株冷害水平最低和30株冷害水平最高的幼苗，构建耐寒池和冷敏感池。使用混合分组分析法（BSA-seq）对这两个池和亲本系进行分析。G'值分析表明C09染色体上的一个单峰为候选区间，该区间有一个2.59 Mb的片段，包含69个候选基因。在七个时间点进行了时间进程和加权基因共表达网络联合分析，以揭示双亲对低温响应的遗传基础。鉴定出12个主要参与植物抗寒的差异表达基因。结合BSA-seq和转录组分析，确定、和为候选基因。候选基因的定量实时PCR验证结果与RNA测序一致。本研究有助于探索油菜的耐寒机制。
