Wu Jinhong, Chen Meiyao, Yang Fangwen, Han Jing, Ma Xiaosong, Li Tianfei, Liu Hongyan, Liang Bin, Yu Shunwu
Shanghai Agrobiological Gene Center, Shanghai 201106, China.
Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai 201106, China.
Plants (Basel). 2025 Jun 5;14(11):1731. doi: 10.3390/plants14111731.
Crop productivity is severely constrained by abiotic and biotic stresses, necessitating innovative strategies to enhance stress resilience. Glycerol-3-phosphate (G3P) is a central metabolite in carbohydrate and lipid metabolism, playing crucial roles in stress responses. In this study, we engineered a novel () gene, designated , by fusing the N-terminal NAD-binding domain of rice with the feedback-resistant C-terminal catalytic domain of . Overexpression of in rice enhanced tolerance to drought, phosphorus deficiency, high temperature, and cadmium (Cd) stresses, while also improving plant growth and yield under drought stress at the adult stage. Notably, the accumulation of glycerol-3-phosphate (G3P) and activities of antioxidant enzymes (SOD, POD, CAT) were significantly elevated in the transgenic plants following osmotic stimuli, and fatty acid profiles were altered, favoring stress adaptation. Transcriptomic analyses revealed that modulates cell wall biogenesis, reactive oxygen species (ROS) scavenging, and lipid metabolism pathways, with minimal disruption to core G3P metabolic genes. These findings highlight the potential of OEGD as a valuable genetic resource for improving stress resistance in rice.
作物生产力受到非生物和生物胁迫的严重制约，因此需要创新策略来增强胁迫抗性。甘油-3-磷酸（G3P）是碳水化合物和脂质代谢中的一种核心代谢物，在胁迫反应中发挥着关键作用。在本研究中，我们通过将水稻[具体基因1]的N端NAD结合结构域与[具体基因2]的反馈抗性C端催化结构域融合，构建了一个新的[目标基因名称]基因，命名为[具体名称]。在水稻中过表达[目标基因名称]可增强对干旱、缺磷、高温和镉（Cd）胁迫的耐受性，同时还能在成年期干旱胁迫下改善植株生长和产量。值得注意的是，在渗透刺激后，转基因植株中甘油-3-磷酸（G3P）的积累和抗氧化酶（SOD、POD、CAT）的活性显著提高，脂肪酸谱发生改变，有利于胁迫适应。转录组分析表明，[目标基因名称]调控细胞壁生物合成、活性氧（ROS）清除和脂质代谢途径，对核心G3P代谢基因的干扰最小。这些发现突出了[目标基因名称]作为提高水稻抗逆性的宝贵遗传资源的潜力。
