Stemshorn B, Nielsen K
Can J Comp Med. 1977 Apr;41(2):152-9.
Selected sera from cattle naturally infected with Brucella abortus precipitate water soluble antigens extracted by sonication from B. abortus. One of these antigens resembles antigen E (Baughn and Freeman) as it is excluded from Sephadex G-200 gels, migrates anodally when electrophoresed at pH 8.6, resists heating at 100 degrees C for ten minutes and appears to be susceptible to papain digestion. Precipitins specific for this antigen remained in sera from which all detectable Brucella agglutinating antibody had been removed by adsorption with live or heat killed B. abortus. The antigen has been extracted from smooth and rough strains of B abortus. Precipitins specific for this antigen have been detected in antisera produced against Brucella canis.
从自然感染牛流产布鲁氏菌的牛中选取的血清，能使通过超声处理从牛流产布鲁氏菌中提取的水溶性抗原沉淀。这些抗原之一类似于抗原E（鲍恩和弗里曼），因为它被葡聚糖G - 200凝胶排除，在pH 8.6条件下电泳时向阳极迁移，能耐受100℃加热10分钟，并且似乎易被木瓜蛋白酶消化。针对该抗原的沉淀素存在于血清中，而这些血清中所有可检测到的布鲁氏菌凝集抗体已通过用活的或热灭活的牛流产布鲁氏菌吸附而去除。该抗原已从牛流产布鲁氏菌的光滑型和粗糙型菌株中提取出来。在针对犬布鲁氏菌产生的抗血清中已检测到针对该抗原的沉淀素。
