Schurig G G, Jones L M, Speth S L, Berman D T
Infect Immun. 1978 Sep;21(3):994-1002. doi: 10.1128/iai.21.3.994-1002.1978.
The smooth lipopolysaccharide complex of the outer surface of smooth Brucella abortus cells is believed to be the antigenic component involved in serological tests routinely used for the diagnosis of brucellosis. Sera from cattle vaccinated or infected with B. abortus generally contain antibody directed toward the smooth lipopolysaccharide complex. The brucella organism contains a large number of other antigenically distinct components. The biological significance of some of these antigens has been demonstrated by showing that sera from infected cattle have precipitins to these components. These sera revealed up to seven distinct lines in immunoelectrophoresis with a protein-rich antigen mixture prepared from rough strain B. abortus 45/20, whereas sera from strain 19-vaccinated cattle did not reveal these lines at 4 or more months after vaccination. Monospecific antisera were prepared against six antigens in this mixture, and the purification of two of them by antibody affinity chromatography is described.
光滑型流产布鲁氏菌细胞外表面的光滑脂多糖复合物被认为是参与布鲁氏菌病诊断常规血清学检测的抗原成分。接种或感染流产布鲁氏菌的牛血清通常含有针对光滑脂多糖复合物的抗体。布鲁氏菌含有大量其他抗原性不同的成分。通过显示感染牛的血清对这些成分有沉淀素，已证明其中一些抗原的生物学意义。这些血清在与由粗糙型流产布鲁氏菌45/20制备的富含蛋白质的抗原混合物进行免疫电泳时显示出多达七条不同的条带，而接种19号菌株的牛血清在接种后4个月或更长时间未显示出这些条带。针对该混合物中的六种抗原制备了单特异性抗血清，并描述了通过抗体亲和层析对其中两种抗原的纯化。
