Gao Yuan, Guo Yuyang, Chen Shurong, Zhang Jianmei, Wang Jinhe, Wang Fuling, Luo Jianghan, Yan Lijun
School of Pharmacy, Engineering Research Center of Natural Antineoplastic Drugs, Ministry of Education, Harbin University of Commerce, Harbin 150076, China.
Molecules. 2025 May 29;30(11):2372. doi: 10.3390/molecules30112372.
This article investigates the antifungal activity of clove essential oil (CEO) against , as well as its inhibitory effects on biofilm formation and the associated developmental processes. Furthermore, it evaluates the therapeutic efficacy of CEO in a mouse model of intestinal infection and explores its impact on intestinal microbiota. Additionally, 16S rRNA high-throughput sequencing was conducted to analyze the alterations in the intestinal microbiota. The findings indicate that the administration of CEO in mice infected with resulted in a normalization of body weight and an improvement in their symptoms. Histological analysis utilizing HE and PAS staining demonstrated that CEO exerted beneficial effects on the intestinal mucosal status of these infected mice. Furthermore, ELISA results revealed a dose-dependent reduction in the levels of IL-6, IL-8, and IL-17A within the small intestinal tissues of -infected mice. Additionally, 16S rRNA gene analysis indicated that CEO effectively enhanced the richness and diversity within the intestinal microbiota of CEO treatment groups of mice that were investigated. Overall, CEO exhibits therapeutic potential against inflammation induced by intestinal infection in mice. This effect can be attributed to its anti-inflammatory properties as well as its capacity to regulate the composition of intestinal flora.
本文研究了丁香精油（CEO）对[具体真菌名称未给出]的抗真菌活性，以及其对生物膜形成和相关发育过程的抑制作用。此外，评估了CEO在小鼠肠道[具体感染名称未给出]感染模型中的治疗效果，并探讨了其对肠道微生物群的影响。另外，进行了16S rRNA高通量测序以分析肠道微生物群的变化。研究结果表明，给感染[具体感染名称未给出]的小鼠施用CEO可使体重恢复正常并改善其症状。利用苏木精-伊红（HE）和过碘酸-雪夫（PAS）染色的组织学分析表明，CEO对这些感染小鼠的肠道黏膜状态产生了有益影响。此外，酶联免疫吸附测定（ELISA）结果显示，感染[具体感染名称未给出]的小鼠小肠组织中白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和白细胞介素-17A（IL-17A）水平呈剂量依赖性降低。另外，16S rRNA基因分析表明，CEO有效提高了所研究的CEO治疗组小鼠肠道微生物群的丰富度和多样性。总体而言，CEO对小鼠肠道[具体感染名称未给出]感染诱导的炎症具有治疗潜力。这种作用可归因于其抗炎特性以及调节肠道菌群组成的能力。
