Petrocelli Giovannamaria, Pampanella Luca, Abruzzo Provvidenza Maria, Cruciani Sara, Ventura Carlo, Canaider Silvia, Facchin Federica
Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Via Massarenti 9, 40138 Bologna, Italy.
Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy.
Molecules. 2025 May 29;30(11):2367. doi: 10.3390/molecules30112367.
Mesenchymal stem cells isolated from human adipose tissue (hASCs) are a promising tool for tissue repair due to their ability to differentiate into specific cell lineages. The possibility of modulating the adipogenic differentiation of hASCs is crucial in improving their therapeutic potential. This study aimed to investigate the effects of cannabidiol (CBD), a phytocannabinoid isolated from L., on hASCs. Few studies have evaluated its role in stem cell (SC) properties and their differentiation potential. hASCs were first treated with different concentrations of CBD (ranging from 0.1 to 10 μM) to assess its effects on viability, demonstrating that this molecule is non-toxic, except at the concentration of 10 μM. Subsequently, the role of CBD in the proliferation, metabolism and adipogenic potential of hASCs was analyzed. CBD promoted adipogenesis in a dose-dependent manner, even in the absence of differentiation medium. This result was evidenced by the presence of lipid vacuoles, the expression of adipogenic markers, cytoskeletal actin rearrangement and modulation in the expression of osteogenic genes. Although the results indicated a role of CBD in promoting hASC adipogenesis, further research will be needed to explore the mechanism of action of CBD in SC differentiation and to deepen its utility in SC-based approaches.
从人脂肪组织中分离出的间充质干细胞（hASCs）因其能够分化为特定细胞谱系，是一种很有前景的组织修复工具。调节hASCs成脂分化的可能性对于提高其治疗潜力至关重要。本研究旨在探讨从大麻中分离出的植物大麻素大麻二酚（CBD）对hASCs的影响。很少有研究评估其在干细胞（SC）特性及其分化潜能中的作用。首先用不同浓度的CBD（范围为0.1至10μM）处理hASCs，以评估其对活力的影响，结果表明该分子无毒，除了在10μM浓度时。随后，分析了CBD在hASCs增殖、代谢和成脂潜能中的作用。即使在没有分化培养基的情况下，CBD也以剂量依赖性方式促进脂肪生成。脂质空泡的存在、成脂标志物的表达、细胞骨架肌动蛋白重排以及成骨基因表达的调节证明了这一结果。尽管结果表明CBD在促进hASC脂肪生成中起作用，但仍需要进一步研究以探索CBD在SC分化中的作用机制，并深化其在基于SC的方法中的效用。
