Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Department of Preventive Dentistry, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou 510182, China.
School and Hospital of Stomatology, Guangzhou Medical University, Guangzhou 510182, China.
Biomolecules. 2023 Jan 6;13(1):118. doi: 10.3390/biom13010118.
Strategies to promote dental pulp stem cells (DPSCs) functions including proliferation, migration, pro-angiogenic effects, and odontogenic/osteogenic differentiation are in urgent need to restore pulpitis-damaged dentin/pulp regeneration and DPSCs-based bone tissue engineering applications. Cannabidiol (CBD), an active component of Cannabis sativa has shown anti-inflammation, chemotactic, anti-microbial, and tissue regenerative potentials. Based on these facts, this study aimed to analyze the effect of CBD on DPSCs proliferation, migration, and osteogenic/odontogenic differentiation in basal and inflammatory conditions. Highly pure DPSCs with characteristics of mesenchymal stem cells (MSCs) were successfully isolated, as indicated by the results of flowcytometry and multi-lineage (osteogenic, adipogenic, and chondrogenic) differentiation potentials. Among the concentration tested (0.1-12.5 µM), CBD (2.5 μM) showed the highest anabolic effect on the proliferation and osteogenic/odontogenic differentiation of DPSCs. Pro-angiogenic growth factor VEGF mRNA expression was robustly higher in CBD-treated DPSCs. CBD also prompted the migration of DPSCs and CBD receptor CB1 and CB2 expression in DPSCs. TNF-α inhibited the viability, migration, and osteogenic/odontogenic differentiation of DPSCs and CBD reversed these effects. CBD alleviated the TNF-α-upregulated expression of pro-inflammatory cytokines TNF-α, interleukin (IL)-1β, and IL-6 in DPSCs. In conclusion, our results indicate the possible application of CBD on DPSCs-based dentin/pulp and bone regeneration.
为了促进牙髓干细胞（DPSCs）的功能，包括增殖、迁移、促血管生成作用和牙源性/成骨分化，急需恢复牙髓炎损伤的牙本质/牙髓再生和基于 DPSCs 的骨组织工程应用。大麻素（Cannabidiol，CBD）是大麻的一种活性成分，具有抗炎、趋化、抗菌和组织再生潜力。基于这些事实，本研究旨在分析 CBD 在基础和炎症条件下对 DPSCs 增殖、迁移和成骨/成牙本质分化的影响。通过流式细胞术和多系（成骨、成脂和成软骨）分化潜能的结果，成功分离出具有间充质干细胞（MSCs）特征的高纯度 DPSCs。在测试的浓度（0.1-12.5 μM）中，CBD（2.5 μM）对 DPSCs 的增殖和成骨/成牙本质分化表现出最高的合成代谢作用。促血管生成生长因子 VEGF 的 mRNA 表达在 CBD 处理的 DPSCs 中更高。CBD 还促使 DPSCs 迁移，以及 DPSCs 中 CBD 受体 CB1 和 CB2 的表达。TNF-α 抑制 DPSCs 的活力、迁移和成骨/成牙本质分化，而 CBD 逆转了这些作用。CBD 减轻了 TNF-α 上调的 DPSCs 中促炎细胞因子 TNF-α、白细胞介素（IL）-1β 和 IL-6 的表达。总之，我们的结果表明 CBD 可能应用于基于 DPSCs 的牙本质/牙髓和骨再生。
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