Li Ruoqing, Yang Xiaojuan, Chen Sheng, Chen Hong, Lin Ling, Chen Yatang, Xiao Jun, Ma Yu, Li Wei
Central Laboratory of Chongqing Emergency Medical Center, Chongqing Key Laboratory of Emergency Medicine, Chongqing Emergency Medical Center, Chongqing University Central Hospital, School of Medicine, Chongqing University, Chongqing 400014, China.
Department of General Medicine, Chongqing Key Laboratory of Emergency Medicine, Chongqing Emergency Medical Center, Chongqing University Central Hospital, School of Medicine, Chongqing University, Chongqing 400014, China.
Nano Lett. 2025 Jun 25;25(25):10225-10232. doi: 10.1021/acs.nanolett.5c02463. Epub 2025 Jun 13.
Biomolecular condensates play important roles in molecular transport within living organisms and have emerged as a promising delivery vehicle for biopharmaceutical applications. Here, we report on the engineering of DNA condensates (DNA droplets) as versatile nanovectors by utilizing their hierarchical structure. Hierarchically structured DNA droplets offer multiple drug loading sites through the utilization of covalent modifications on the primary structure (phosphate backbone), noncovalent interactions on the secondary structure (grooves), or physical retention on higher-order structure (pores). We found that DNA droplets are capable of loading small molecules and amino-based cargo through noncovalent or covalent interactions. The spatial position of different cargos loaded onto DNA droplets can be precisely controlled. Additionally, DNA droplets can effectively scavenge reactive oxygen species. This study demonstrates that DNA droplets can provide multiple drug loading sites and precisely control the positions of different drugs, making DNA droplets a promising functional nanovector for drug delivery.
生物分子凝聚物在生物体内的分子运输中发挥着重要作用，并已成为生物制药应用中一种很有前景的递送载体。在此，我们报告了通过利用DNA凝聚物（DNA液滴）的层次结构将其工程化为通用纳米载体的研究。分层结构的DNA液滴通过利用一级结构（磷酸骨架）上的共价修饰、二级结构（凹槽）上的非共价相互作用或高阶结构（孔隙）上的物理保留提供多个药物负载位点。我们发现DNA液滴能够通过非共价或共价相互作用负载小分子和氨基类货物。负载在DNA液滴上的不同货物的空间位置可以得到精确控制。此外，DNA液滴能够有效清除活性氧。这项研究表明，DNA液滴可以提供多个药物负载位点并精确控制不同药物的位置，使DNA液滴成为一种很有前景的用于药物递送的功能性纳米载体。
