Xie Guangchao, Li Bingjie, Guo Shuyue, Hou Wenjing, Li Molin, Wang Yao, Liu Jun, Hao Jihui, Wei Xi
Department of Diagnostic and Therapeutic Ultrasonography, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, Tianjin's Clinical Research Center for Cancer, Tianjin Key Laboratory of Digestive Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China.
Department of Radiology and Tianjin Key Laboratory of Functional Imaging, Tianjin Medical University General Hospital, Tianjin, 300052, China.
Mater Today Bio. 2025 May 26;32:101910. doi: 10.1016/j.mtbio.2025.101910. eCollection 2025 Jun.
Combined therapy based on magnetic hyperthermia holds significant promise for tumor treatment. Nevertheless, the existing magnetic hyperthermia platforms are often constrained by suboptimal heating efficiency and concerns over the potential metal ion toxicity. Herein, a minimalistic and nonmetallic graphite-agarose gel-drug (Gra-Aga-Drug) implant is prepared by mold-assisted casting method for magnetic hyperthermia-based combination therapy of tumors. This implant can achieve magnetic hyperthermia through the strong eddy current thermal effect of Gra, while the agarose gel provides the implant with universal and efficient drug-loading capacity and magnetic hyperthermia-responsive release ability. The chemotherapeutic drug doxorubicin (DOX) and the immune adjuvant imiquimod (R837) are chosen to prepare Gra-Aga-DOX-R837 implant for combination therapy of tumors . The magnetic hyperthermia and DOX induce immunogenic cell death (ICD) of tumors, combined with the immune adjuvant R837, eliciting a robust anti-tumor immune response to efficiently ablate primary tumors and suppress distant tumors growth. Importantly, the implant platform effectively addresses the challenges of limited heating efficiency and metal ion toxicity associated with traditional magnetic hyperthermia materials. This study marks the pioneering construction of nonmetallic combination therapy platform with excellent magnetothermal performance and favorable biocompatibility.
基于磁热疗的联合疗法在肿瘤治疗方面具有巨大潜力。然而，现有的磁热疗平台常常受到加热效率欠佳以及对潜在金属离子毒性担忧的限制。在此，通过模具辅助浇铸法制备了一种简约的非金属石墨-琼脂糖凝胶-药物（Gra-Aga-Drug）植入物，用于基于磁热疗的肿瘤联合治疗。这种植入物可通过Gra的强涡电流热效应实现磁热疗，而琼脂糖凝胶为植入物提供了通用且高效的载药能力以及磁热疗响应释放能力。选用化疗药物阿霉素（DOX）和免疫佐剂咪喹莫特（R837）制备Gra-Aga-DOX-R837植入物用于肿瘤联合治疗。磁热疗和DOX诱导肿瘤发生免疫原性细胞死亡（ICD），与免疫佐剂R837相结合，引发强大的抗肿瘤免疫反应，以有效消融原发性肿瘤并抑制远处肿瘤生长。重要的是，该植入物平台有效解决了与传统磁热疗材料相关的加热效率有限和金属离子毒性的挑战。本研究标志着具有优异磁热性能和良好生物相容性的非金属联合治疗平台的开创性构建。
