Evaluate the Antimicrobial Properties of Double Antibiotic Paste (DAP) and Morinda Citrifolia and Propolis Paste (MCP) Used in Regenerative Endodontics: An Study.

OBJECTIVE

To Evaluate The Antimicrobial Properties of Double Antibiotic Paste and And Propolis Paste Used In Regenerative Endodontics.

MATERIALS AND METHOD

Using intact human teeth, radicular dentin samples were prepared (dimensions 4×4×2 mm) by following a standard method. The samples were cut using a diamond saw at minimal speed using water as coolant and smoothed with abrasive papers. To expose the dentin tubules and eliminate the smear layer, they were treated with NaOCl (1.5%), distilled water, and EDTA (17%). A total of 30 dentin samples went through a three-week anaerobic infection with bacterial biofilms sourced from the root canal of an underdeveloped tooth showing pulp necrosis. Group 1 acted as the control, while Group 2 received treatment with DAP, and Group 3 was treated with a paste of and Propolis. After a week, pastes were cleaned, and specimens were dipped in phosphate-buffered saline for a day. The biofilm was released from each specimen by sonicating and vortexing it for 30 seconds. It was then diluted, spiral-plated on blood agar, and cultured anaerobically for a day. An automated colony counter was utilized to measure colony-forming units (CFU/mL), and statistical analysis was conducted using the Wilcoxon rank-sum test.

RESULT

The wash from dentin samples treated with DAP and MCP showed minimal CFU counts, indicating both pastes' high efficiency against biofilm-forming microbes from the canals of an immature tooth exhibiting pulp necrosis.

CONCLUSION

Dentin samples infused with DAP and MCP showed minimal CFU counts, indicating their high effectiveness against biofilm-forming microbes from the root canal of an underdeveloped tooth with pulp necrosis. Additional research is required to validate these findings.