Xu Zhi, Jin Yile, Zhang Zhihui, HePeng Yixiang, Zhang Chen, Yang Surui, Qu Kailin, Ning Qi, Kourouma Mory, Liu Ruiyuan, Wu Xu, Li Xiang
Huiqiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
Department of General Practice, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
J Mater Chem B. 2025 Jul 10;13(27):8208-8218. doi: 10.1039/d5tb00716j.
Photodynamic therapy (PDT) has emerged as a promising treatment for drug-resistant bacterial infections by avoiding the abuse of antibiotics. However, most PDTs rely on reactive oxygen species (ROS) generated a type II process, which limits the antimicrobial effect in a hypoxic microenvironment. Herein, we reported phycocyanin functionalized selenium nanoparticles (Se@PC NPs) for type I photodynamic antibacterial therapy and wound healing. Se@PC NPs can generate hydroxyl radicals and superoxide radicals under visible light irradiation, effectively disrupting the bacterial membrane structure and demonstrating sterilization against Gram-positive and Gram-negative bacteria. Notably, experiments and histological tests have demonstrated that Se@PC NPs effectively eliminate bacteria, regulate proinflammatory cytokines against bacteria-induced inflammation, promote collagen deposition, and accelerate wound healing. Consequently, this study provides a strategy for the design of highly effective type I photosensitizers for photodynamic antibacterial therapy.
光动力疗法（PDT）已成为一种通过避免抗生素滥用治疗耐药细菌感染的有前景的方法。然而，大多数光动力疗法依赖于II型过程产生的活性氧（ROS），这限制了其在缺氧微环境中的抗菌效果。在此，我们报道了用于I型光动力抗菌治疗和伤口愈合的藻蓝蛋白功能化硒纳米颗粒（Se@PC NPs）。Se@PC NPs在可见光照射下可产生羟基自由基和超氧自由基，有效破坏细菌膜结构，并对革兰氏阳性菌和革兰氏阴性菌表现出杀菌作用。值得注意的是，实验和组织学测试表明，Se@PC NPs能有效消除细菌，调节针对细菌诱导炎症的促炎细胞因子，促进胶原蛋白沉积，并加速伤口愈合。因此，本研究为设计用于光动力抗菌治疗的高效I型光敏剂提供了一种策略。
