Jiang Jieming, Zhu Qiuhua, Li Yang, Wu Jiayuan, Zou Chenghuan, Li Hongbo, Lei Xiaohui, Zhang Fangyuan, Lin Yujie, Cao Rui, Cao Ying, Mo Jiawang, Lai Jianbin, Zhao Jiangzhe, Wang Chao, Yang Chengwei
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou 510631, China.
College of Life Sciences, Shaoxing University, Shaoxing 312000, China.
Sci Adv. 2025 Jun 13;11(24):eadt2842. doi: 10.1126/sciadv.adt2842.
DNA damage arises from various environmental stresses, and ABA is well known for its roles in plant stress resistance. However, its function in plant DNA damage tolerance remains unclear. In this study, we showed that ABA supplementation significantly enhances plant tolerance to DNA-damaging treatments. SnRK2.2 and SnRK2.3 kinases in the ABA signaling pathway are pivotal in this process. These kinases interact with clathrin light chain 2 (CLC2), facilitating its phosphorylation and nuclear translocation in response to Zeocin and ABA treatment. In the nucleus, CLC2 interacts with ADA2b, an adaptor protein crucial for recruiting SMC5/6 complex to the double-strand break (DSB) sites. The enhanced nuclear localization of CLC2 is essential for the accurate localization of ADA2b at DSB sites. Collectively, our study uncovers that ABA enhances plant DNA damage tolerance with a distinct function of CLC2 in genomic stability maintaining, thereby improving our understanding of DNA damage tolerance mechanisms in plants.
DNA损伤源于各种环境胁迫，而脱落酸（ABA）在植物抗逆性中的作用广为人知。然而，其在植物DNA损伤耐受性方面的功能仍不清楚。在本研究中，我们发现补充ABA能显著增强植物对DNA损伤处理的耐受性。ABA信号通路中的SnRK2.2和SnRK2.3激酶在此过程中起关键作用。这些激酶与网格蛋白轻链2（CLC2）相互作用，促进其磷酸化并响应博来霉素和ABA处理发生核转位。在细胞核中，CLC2与ADA2b相互作用，ADA2b是一种衔接蛋白，对于将SMC5/6复合体招募到双链断裂（DSB）位点至关重要。CLC2核定位的增强对于ADA2b在DSB位点的准确定位至关重要。总的来说，我们的研究揭示了ABA通过CLC2在维持基因组稳定性方面的独特功能增强了植物的DNA损伤耐受性，从而增进了我们对植物DNA损伤耐受机制的理解。
