Khomyakova M A, Merkel A Y, Novikov A A, Slobodkin A I
Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Leninskiy Prospect, 33, bld. 2, 119071, Moscow, Russia.
Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Leninskiy Prospect, 33, bld. 2, 119071, Moscow, Russia.
Syst Appl Microbiol. 2025 Jul;48(4):126629. doi: 10.1016/j.syapm.2025.126629. Epub 2025 Jun 10.
A novel anaerobic free-living bacterium (strain C05PYG) was isolated from a terrestrial mud volcano (Taman Peninsula, Russia). Cells were pleomorphic but mainly consisted of small, cell wall-less, non-motile cocci, 0.40-0.46 μm in diameter. The temperature range for growth was 5-37 °C (optimum at 30 °C). The pH range for growth was 7.5-10.0, with an optimum at pH 8.0-9.0. It was a chemoorganoheterotroph, fermenting mono-, di- and polysaccharides (dextrin, starch), proteinaceous substrates and organic acids (lactate and pyruvate). Yeast extract was essential for growth. Strain C05PYG possessed an ability to degrade extraneous DNA. Major cellular fatty acids were C and i-C; major dimethyl acetals were C DMA and i-C DMA. The genome of strain C05PYG had a size of 2.0 Mb with a DNA G + C content of 33.0 % (WGS). Phylogenetic analysis based on 120 marker genes placed strain C05PYG in a separate order adjacent to the order Acholeplasmatales within the class Mollicutes, hitherto referred to as "Izemoplasmatales", which contains no representatives with validly published names. We propose to assign strain C05PYG to a novel species of a novel genus, Liberiplasma polymorphum gen. nov., sp. nov.; the type strain is C05PYG (=VKM B-3767 = BIM B-2060 = UQM 41800). This genus is placed in a novel family, Liberiplasmataceae fam. nov. within the novel order Liberiplasmatales ord. nov., class Mollicutes.
从俄罗斯塔曼半岛的陆地泥火山中分离出一种新型厌氧自由生活细菌（菌株C05PYG）。细胞呈多形性，但主要由直径为0.40 - 0.46μm的小型、无细胞壁、不运动的球菌组成。生长温度范围为5 - 37°C（最适温度为30°C）。生长的pH范围为7.5 - 10.0，最适pH为8.0 - 9.0。它是一种化能有机异养菌，能发酵单糖、二糖和多糖（糊精、淀粉）、蛋白质底物和有机酸（乳酸和丙酮酸）。酵母提取物对生长至关重要。菌株C05PYG具有降解外源DNA的能力。主要细胞脂肪酸为C和i - C；主要二甲基缩醛为C DMA和i - C DMA。菌株C05PYG的基因组大小为2.0 Mb，DNA G + C含量为33.0%（全基因组测序）。基于120个标记基因的系统发育分析将菌株C05PYG置于柔膜菌纲内与无胆甾原体目相邻的一个单独目，迄今称为“伊泽莫原体目”，该目中没有有效发表名称的代表菌株。我们提议将菌株C05PYG归入一个新属的新物种，多形自由原体属（Liberiplasma polymorphum），新属，新种；模式菌株为C05PYG（=VKM B - 3767 = BIM B - 2060 = UQM 41800）。该属置于新目自由原体目（Liberiplasmatales）内的一个新科自由原体科（Liberiplasmataceae），柔膜菌纲。
