Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
Int J Syst Evol Microbiol. 2024 Jul;74(7). doi: 10.1099/ijsem.0.006447.
A Gram-stain-negative, yellow-pigmented, and facultatively aerobic bacterium, designated strain GPA1, was isolated from plastic waste landfill soil in the Republic of Korea. The cells were non-motile short rods exhibiting oxidase-negative and catalase-positive activities. Growth was observed at 15-40 °C (optimum, 30 °C), at pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 0-2.5 % (w/v) NaCl (optimum, 0 %). Menaquinone-7 was the sole respiratory quinone, and iso-C, C 5, and iso-C 3-OH were the major cellular fatty acids (>10 % of the total fatty acids). Phosphatidylethanolamine was identified as a major polar lipid. Phylogenetic analyses based on 16S rRNA gene sequences and 120 concatenated marker protein sequences revealed that strain GPA1 formed a distinct lineage within the genus . The genome of strain GPA1 was 6078 kb in size with 53.8 mol% G+C content. Strain GPA1 exhibited the highest similarity to T16R-86, with a 98.6 % 16S rRNA gene sequence similarity, but their average nucleotide identity and digital DNA-DNA hybridization values were 82.5 and 25.9 %, respectively. Based on its phenotypic, chemotaxonomic, and phylogenetic characteristics, strain GPA1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GPA1 (=KACC 23415=JCM 36644).
一株革兰氏阴性、黄色着色、兼性需氧细菌,被命名为 GPA1 菌株,从韩国的塑料垃圾填埋场土壤中分离得到。细胞为非运动性短杆菌,氧化酶阴性,过氧化氢酶阳性。在 15-40°C(最佳温度 30°C)、pH 6.0-9.0(最佳 pH 7.0-8.0)和 0-2.5%(w/v)NaCl(最佳 0%)的条件下可以生长。menaquinone-7 是唯一的呼吸醌,iso-C、C 5 和 iso-C 3-OH 是主要的细胞脂肪酸(占总脂肪酸的>10%)。磷脂酰乙醇胺被鉴定为主要的极性脂。基于 16S rRNA 基因序列和 120 个串联标记蛋白序列的系统发育分析表明,菌株 GPA1 在 属内形成一个独特的分支。菌株 GPA1 的基因组大小为 6078 kb,G+C 含量为 53.8 mol%。菌株 GPA1 与 T16R-86 的 16S rRNA 基因序列相似度最高,为 98.6%,但其平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 82.5%和 25.9%。根据其表型、化学分类和系统发育特征,菌株 GPA1 代表了 属的一个新种,建议将其命名为. sp. nov.。模式菌株为 GPA1(=KACC 23415=JCM 36644)。