Peng Hanyu, Tan Jun, Li Xiao
Department of Stomatology, Affiliated Hospital of North Sichuan Medical College, Nanchong, People's Republic of China.
School of Stomatology, Southern Medical University, Guangzhou, People's Republic of China.
Biomed Mater. 2025 Jul 1;20(4). doi: 10.1088/1748-605X/ade488.
Curcumin is a natural polyphenolic compound derived from turmeric, which exhibits a wide range of pharmacological activities, including anti-inflammatory and promoting bone healing effects. To enhance the bioactivity of the surface of titanium implants and promote early bone integration, the pure titanium surface was modified by composite modification through electrochemical anodic oxidation and drug coating. The surface of the prepared materials was characterized by scanning electron microscopy, atomic force microscopy, x-ray photoelectron spectroscopy, and surface contact angle analyzer. The drug release performance of the modified titanium surfaces was evaluated by ultraviolet spectrophotometry. Rat bone marrow mesenchymal stem cells (BMSCs) were extracted and identified. The effects of surface modification on cell viability were investigated through CCK-8, cell adhesion, and live/dead cell staining experiments. The effects of different surface-treated titanium sheets on osteogenic differentiation of BMSCs were evaluated by transwell assay, alkaline phosphatase activity assay, reverse transcription quantitative polymerase chain reaction, and mineralization nodule staining experiments. The results showed that successful loading of TiOnanotubes with curcumin was prepared, and the surface-modified titanium sheets had effective physical properties (excellent corrosion resistance, mechanical properties and hydrophilicity) and drug release capabilities. The results ofcell culture experiments indicated that superior cell adhesion morphology was observed on the surface of each group of titanium sheets. TiOnanotubes and curcumin could significantly promote BMSCs proliferation and showed pleasant biocompatibility. Theosteogenic induction differentiation experiments confirmed that the TiOnanotube structure and curcumin coating could promote osteogenic differentiation of BMSCs. This study provides a significant theoretical foundation and experimental support for the development of bioactive implants for dental applications.
姜黄素是一种从姜黄中提取的天然多酚化合物，具有广泛的药理活性，包括抗炎和促进骨愈合的作用。为了增强钛植入物表面的生物活性并促进早期骨整合，通过电化学阳极氧化和药物涂层复合改性对纯钛表面进行了修饰。通过扫描电子显微镜、原子力显微镜、X射线光电子能谱和表面接触角分析仪对制备材料的表面进行了表征。采用紫外分光光度法评价改性钛表面的药物释放性能。提取并鉴定大鼠骨髓间充质干细胞（BMSCs）。通过CCK-8、细胞黏附及活/死细胞染色实验研究表面改性对细胞活力的影响。通过Transwell实验、碱性磷酸酶活性测定、逆转录定量聚合酶链反应和矿化结节染色实验评价不同表面处理钛片对BMSCs成骨分化的影响。结果表明，成功制备了负载姜黄素的TiO纳米管，表面改性钛片具有有效的物理性能（优异的耐腐蚀性、力学性能和亲水性）和药物释放能力。细胞培养实验结果表明，每组钛片表面均观察到优异的细胞黏附形态。TiO纳米管和姜黄素能显著促进BMSCs增殖，具有良好的生物相容性。成骨诱导分化实验证实，TiO纳米管结构和姜黄素涂层能促进BMSCs的成骨分化。本研究为牙科应用生物活性植入物的开发提供了重要的理论基础和实验支持。
