Li Shaoheng, Kner Peter
Opt Express. 2024 Sep 23;32(20):35406-35418. doi: 10.1364/OE.535429.
Self-interference digital holography (SIDH) enables 3D imaging of incoherently emitting objects over a large axial range using only three 2D images. Our previous research demonstrated that point-like sources emitting as few as 4,200 photons can be reconstructed over a 10 µm axial range. Combining SIDH with single-molecule localization microscopy (SMLM) has the potential to achieve 3D super-resolution imaging across a large axial range without mechanical refocusing of the sample. However, optical aberrations affect the localization performance of SIDH and must be corrected, especially for large-volume 3D imaging. In this paper, we propose a fast, guide-star-free computational aberration correction method for SIDH. Our method can correct optical aberrations in low signal light conditions over the entire imaging axial range without any additional hardware. We use a sensorless-AO method in a virtual pupil plane to optimize the wavefront based on a frequency-space metric. Using this method, we demonstrate an improvement in both the Strehl ratio up to ∼0.98 and the SIDH localization precision to near the ideal case.
自干涉数字全息术（SIDH）仅使用三张二维图像就能在大轴向范围内对非相干发射物体进行三维成像。我们之前的研究表明，对于发射光子数低至4200个的点状光源，能够在10 µm的轴向范围内进行重建。将SIDH与单分子定位显微镜（SMLM）相结合，有潜力在无需对样品进行机械重新聚焦的情况下，在大轴向范围内实现三维超分辨率成像。然而，光学像差会影响SIDH的定位性能，必须进行校正，特别是对于大体积三维成像。在本文中，我们提出了一种用于SIDH的快速、无引导星的计算像差校正方法。我们的方法可以在整个成像轴向范围内的低信号光条件下校正光学像差，而无需任何额外硬件。我们在虚拟光瞳平面中使用无传感器自适应光学方法，基于频率空间度量来优化波前。使用这种方法，我们证明了斯特列尔比提高到了约0.98，并且SIDH定位精度接近理想情况。
