基于自干涉数字全息术的三维光学定位显微镜的基本精度界限
Fundamental precision bounds for three-dimensional optical localization microscopy using self-interference digital holography.
作者信息
Marar Abhijit, Kner Peter
机构信息
School of Electrical and Computer Engineering, University of Georgia, Athens, Georgia 30602, USA.
出版信息
Biomed Opt Express. 2020 Dec 3;12(1):20-40. doi: 10.1364/BOE.400712. eCollection 2021 Jan 1.
Localization based microscopy using self-interference digital holography (SIDH) provides three-dimensional (3D) positional information about point sources with nanometer scale precision. To understand the performance limits of SIDH, here we calculate the theoretical limit to localization precision for SIDH when designed with two different configurations. One configuration creates the hologram using a plane wave and a spherical wave while the second configuration creates the hologram using two spherical waves. We further compare the calculated precision bounds to the 3D single molecule localization precision from different Point Spread Functions. SIDH results in almost constant localization precision in all three dimensions for a 20 m thick depth of field. For high signal-to-background ratio (SBR), SIDH on average achieves better localization precision. For lower SBR values, the large size of the hologram on the detector becomes a problem, and PSF models perform better.
使用自干涉数字全息术（SIDH）的基于定位的显微镜能够以纳米级精度提供关于点光源的三维（3D）位置信息。为了理解SIDH的性能极限，我们在此计算了采用两种不同配置设计时SIDH定位精度的理论极限。一种配置使用平面波和球面波创建全息图，而第二种配置使用两个球面波创建全息图。我们进一步将计算出的精度界限与来自不同点扩散函数的3D单分子定位精度进行比较。对于20微米厚的景深，SIDH在所有三个维度上都能实现几乎恒定的定位精度。对于高信噪比（SBR），SIDH平均能实现更好的定位精度。对于较低的SBR值，探测器上全息图的大尺寸会成为一个问题，此时点扩散函数（PSF）模型表现更好。
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