Manlapig Jamal James D, Crisostomo Jane Camille A, Kondo Makoto, Ban-Tokuda Tomomi, Matsui Hiroki
Department of Animal Science, College of Agriculture, Central Luzon State University, Science City of Muñoz, Philippines.
Graduate School of Bioresources, Mie University, Tsu, Japan.
J Sci Food Agric. 2025 Oct;105(13):7362-7368. doi: 10.1002/jsfa.14437. Epub 2025 Jun 13.
Livestock production contributes largely to climate change and methane (CH) from enteric fermentation is the primary greenhouse gas emitted from ruminants. Thus, developing effective strategies to reduce the impact of ruminant production on the environment is crucial, and the combination of CH mitigating agents may be a viable strategy to attain this. The present study aimed to evaluate the effect of rice bran alone (control+rice bran, RB) or in combination with acrylate (control+rice bran+acrylate, AA) or pyruvate (control+rice bran+pyruvate, PA) on rumen fermentation parameters, CH production, and microbial populations in vitro.
Gas production was highest in control + rice bran + pyruvate (PA) treatments and lowest (P < 0.01) in control + rice bran + acrylate (AA), whereas dry matter digestibility (DMD) was significantly lower (P < 0.05) in AA compared to control (CON) and PA. CH and CH/digested dry matter decreased (P < 0.05) by more than 66% and 63%, respectively, in AA cultures, whereas total organic acid production increased (P < 0.01) compared to CON. Lactate levels were significantly higher (P < 0.05), while acetate, propionate and butyrate were notably lower (P < 0.05) in AA cultures than CON. Microbial analysis showed a significant decrease in methanogen populations in AA than CON (P < 0.01). Compared to CON, anaerobic fungi and Megasphaera elsdenii populations declined across all treatments (P < 0.01), whereas Selenomonas ruminantium populations decreased in RB and AA, and Prevotella ruminicola populations were higher in RB and PA (P < 0.05).
These findings suggest that incorporating RB with AA could be a viable CH inhibitor; however, because of its negative effect on gas production and DMD, further investigations should be done to determine its optimum inclusion level. © 2025 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
畜牧业在很大程度上导致气候变化，反刍动物肠道发酵产生的甲烷（CH₄）是反刍动物排放的主要温室气体。因此，制定有效的策略以减少反刍动物生产对环境的影响至关重要，而甲烷减排剂的组合可能是实现这一目标的可行策略。本研究旨在评估单独使用米糠（对照+米糠，RB）或与丙烯酸盐（对照+米糠+丙烯酸盐，AA）或丙酮酸盐（对照+米糠+丙酮酸盐，PA）组合对体外瘤胃发酵参数、甲烷产生和微生物种群的影响。
对照+米糠+丙酮酸盐（PA）处理的产气量最高，对照+米糠+丙烯酸盐（AA）处理的产气量最低（P＜0.01），而与对照（CON）和PA相比，AA处理的干物质消化率（DMD）显著降低（P＜0.05）。在AA培养物中，CH₄和CH₄/消化干物质分别降低（P＜0.05）超过66%和63%，而与CON相比，总有机酸产量增加（P＜0.01）。AA培养物中的乳酸水平显著更高（P＜0.05），而乙酸、丙酸和丁酸水平显著低于CON（P＜0.05）。微生物分析表明，AA中的产甲烷菌数量比CON显著减少（P＜0.01）。与CON相比，所有处理中的厌氧真菌和埃氏巨球型菌数量均下降（P＜0.01），而RB和AA中的反刍月形单胞菌数量减少，RB和PA中的栖瘤胃普雷沃氏菌数量更高（P＜0.05）。
这些发现表明，将RB与AA结合可能是一种可行的CH₄抑制剂；然而，由于其对产气量和DMD有负面影响，应进一步研究以确定其最佳添加水平。© 2025作者。《食品与农业科学杂志》由约翰·威利父子有限公司代表化学工业协会出版。
