含t-SNARE结构域的大豆胞囊线虫HgSNARE1是一种效应蛋白，它与GmSNAP18-GmSHMT08-GmPR1-15模块相互作用，并对大豆表现出无毒力。
The t-SNARE domain-containing soybean cyst nematode HgSNARE1 is an effector that interacts with the GmSNAP18-GmSHMT08-GmPR1-15 module and shows avirulence to soybean.
作者信息
Zhang Liuping, Zhao Jie, Kong Lingan, Huang Wenkun, Peng Huan, Peng Deliang, Yu Feng, Liu Shiming
机构信息
Hunan Research Center of the Basic Discipline for Cell Signaling, College of Biology, Hunan University, Changsha, People's Republic of China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
出版信息
Pest Manag Sci. 2025 Oct;81(10):6416-6426. doi: 10.1002/ps.8978. Epub 2025 Jun 14.
BACKGROUND
Soybean cyst nematode (SCN, Heterodera glycines) poses a significant threat to soybean (Glycine max (L.)), causing huge yield losses annually in global soybean production. Although the two main soybean SCN-resistant genes, rhg1 GmSNAP18 and Rhg4 GmSHMT08, have been cloned, the SCN effectors have been identified and functioned rarely, and the molecular mechanisms of effectors interacting with soybean remain largely unknown. Here, we identified the SCN effector HgSNARE1 and investigated how it targeted the soybean GmSNAP18-GmSHMT08-GmPR1-15 module, aiming to reveal a new molecular mechanism for SCN control.
RESULTS
In this study, the t-SNARE domain-containing protein HgSNARE1 was identified as an effector from SCN that was expressed the highest at the third juvenile (J3) stage. Expression of HgSNARE1 in soybean hairy roots promoted SCN resistance, indicating the avirulence function of HgSNARE1 to soybean. HgSNARE1 interacted with GmSNAP18 via the t-SNARE domain, with the last five amino acid residues (SVHKA) in the C terminus playing an essential role in the interaction. GmSNAP18 interacted with both GmSHMT08 and a pathogenesis-related (PR) 1 protein GmPR1-15 (Glyma.15 g062400), and GmSHMT08 interacted with GmPR1-15. Therefore, GmSNAP18, GmSHMT08 and GmPR1-15 formed a module by pairwise interactions. However, neither GmSHMT08 nor GmPR1-15 interacted with HgSNARE1. Furthermore, expression of HgSNARE1 in soybean hairy roots stimulated expression of GmPR1-15.
CONCLUSION
These findings reveal a novel molecular mechanism by which the SCN effector HgSNARE1 interacts with SCN-resistant GmSNAP18 of the GmSNAP18-GmSHMT08-GmPR1-15 module and promotes GmPR1-15 expression to confer avirulence, providing a potential target for the development of new SCN-resistant germplasms, and innovative nematode management strategies. © 2025 Society of Chemical Industry.
背景
大豆胞囊线虫（SCN，大豆异皮线虫）对大豆（Glycine max (L.)）构成重大威胁，每年在全球大豆生产中造成巨大产量损失。尽管已克隆出两个主要的大豆抗SCN基因rhg1 GmSNAP18和Rhg4 GmSHMT08，但SCN效应子已被鉴定出来且功能鲜为人知，效应子与大豆相互作用的分子机制仍 largely 未知。在此，我们鉴定了SCN效应子HgSNARE1，并研究了它如何靶向大豆GmSNAP18 - GmSHMT08 - GmPR1 - 15模块，旨在揭示一种新的SCN防治分子机制。
结果
在本研究中，含t - SNARE结构域的蛋白HgSNARE1被鉴定为来自SCN的效应子，在第三龄幼虫（J3）阶段表达最高。HgSNARE1在大豆毛状根中的表达促进了对SCN的抗性，表明HgSNARE1对大豆具有无毒功能。HgSNARE1通过t - SNARE结构域与GmSNAP18相互作用，C末端的最后五个氨基酸残基（SVHKA）在相互作用中起关键作用。GmSNAP18与GmSHMT08和一种病程相关（PR）1蛋白GmPR1 - 15（Glyma.15 g062400）相互作用，且GmSHMT08与GmPR1 - 15相互作用。因此，GmSNAP18、GmSHMT08和GmPR1 - 15通过两两相互作用形成一个模块。然而，GmSHMT08和GmPR1 - 15均不与HgSNARE1相互作用。此外，HgSNARE1在大豆毛状根中的表达刺激了GmPR1 - 15的表达。
结论
这些发现揭示了一种新的分子机制，即SCN效应子HgSNARE1与GmSNAP18 - GmSHMT08 - GmPR1 - 15模块中的抗SCN的GmSNAP18相互作用，并促进GmPR1 - 15表达以赋予无毒功能，为开发新的抗SCN种质和创新的线虫管理策略提供了潜在靶点。© 2025化学工业协会。
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