Hodzi Pride, Masunda Blessed, Mutibvu Tonderai, Charambira Takudzwa, Mafigu Takudzwa, Nhara Rumbidzai
Department of Livestock Sciences, University of Zimbabwe, P.O Box 167 MP, Mount Pleasant Harare, Zimbabwe.
Faculty of Agriculture, Zimbabwe Open University, Corner House, Harare, Zimbabwe.
Trop Anim Health Prod. 2025 Jun 14;57(5):269. doi: 10.1007/s11250-025-04528-y.
This study evaluated Boschveld chicken's antibody response to a standard Newcastle Disease (ND) vaccination program designed for the Hyline Brown chickens. Both breeds were challenged with the LaSota vaccine at days 1, 69, 111, and 195. The blood samples were aseptically collected from 10 randomly selected birds per breed on each vaccination day (1, 69, 111, and 195) and 10 days after each vaccination (10 dpi). The sera were tested for anti-ND virus (NDV) antibodies using the Haemagglutination inhibition (HI) assay. The anti-NDV antibody titers were expressed in log as geometric mean ± standard deviation. Before any vaccination, 80% of Boschveld chicken sera tested negative for anti-ND virus antibodies, significantly higher than that of Hyline Brown chickens (40%). Hyline Brown day-old chicks had a higher antibody titer (4.95 ± 0.21) than Boschveld day-old chicks (3.21 ± 0.43) before vaccination. The Boschveld accumulated higher antibody titers (13.98) than the Hyline Brown breed (13.85), despite the Boschveld having lower antibody titers at day 1. The antibody titers of the Boschveld did not change significantly (p > 0.05) after the 4th vaccination dose. For effective immune control of ND, the vaccination program for commercial layer chickens can be optimized to suit the Boschveld chickens by reducing the frequency of booster vaccinations. The 4th ND vaccination must be delayed until antibody titers approach the minimum protective threshold. This intervention can also be adopted for Boschveld chicken's close relatives, such as village (native) chickens.
本研究评估了博施费尔德鸡对为海兰褐鸡设计的标准新城疫（ND）疫苗接种程序的抗体反应。两个品种均在第1、69、111和195天用LaSota疫苗进行攻毒。在每个接种日（第1、69、111和195天）以及每次接种后10天（接种后10天），从每个品种中随机选取10只鸡无菌采集血样。使用血凝抑制（HI）试验检测血清中的抗新城疫病毒（NDV）抗体。抗NDV抗体滴度以对数形式表示为几何平均值±标准差。在任何疫苗接种之前，80%的博施费尔德鸡血清抗ND病毒抗体检测呈阴性，显著高于海兰褐鸡（40%）。接种疫苗前，海兰褐雏鸡的抗体滴度（4.95±0.21）高于博施费尔德雏鸡（3.21±0.43）。尽管博施费尔德鸡在第1天时抗体滴度较低，但其积累的抗体滴度（13.98）高于海兰褐品种（13.85）。在第4剂疫苗接种后，博施费尔德鸡的抗体滴度没有显著变化（p>0.05）。为了对新城疫进行有效的免疫控制，可以通过减少加强免疫的频率来优化商业蛋鸡的疫苗接种程序，以适应博施费尔德鸡。第4次新城疫疫苗接种必须推迟到抗体滴度接近最低保护阈值时进行。这种干预措施也可应用于博施费尔德鸡的近亲，如乡村（本地）鸡。
