Świecimska Magdalena, Golinska Patrycja, Sangal Vartul, Wachnowicz Bartosz, Goodfellow Michael
Department of Microbiology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, Lwowska 1, 87 100 Torun, Poland.
Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne, UK.
Int J Syst Evol Microbiol. 2023 Jul;73(7). doi: 10.1099/ijsem.0.005978.
Filamentous actinomycetes, designated SL13 and SL54, were isolated from pine litter and their taxonomic status resolved using a polyphasic approach. The isolates exhibit chemotaxonomic and morphological properties consistent with their classification in the family . They form extensively branched substrate mycelia bearing aerial hyphae that differentiate into straight chains of cylindrical spores. The whole-organism hydrolysates contain ll-diaminopimelic acid, glucose, mannose and ribose, the predominant isoprenologue is MK-9(H), the polar lipids are diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and glycophospholipids, and the major fatty acids are anteiso-C, iso-C, iso-C and anteiso-C. Phylogenetic trees based on 16S rRNA gene sequences and multilocus gene sequences of conserved housekeeping genes show that the isolates form a well-supported lineage that is most closely related to NBRC 115203. All of these strains form a well-defined clade in the multilocus sequence analysis tree together with DSM 46488, DSM 41836 and DSM 42083. Draft genomes assemblies of the isolates are rich in biosynthetic gene clusters predicted to produce novel specialized metabolites and stress-related genes which provide an insight into how they have adapted to the harsh conditions that prevail in pine litter. Phylogenomically, both isolates belong to the same lineage as the type strains of , , and ; these relationships are underpinned by high average amino acid identity, average nucleotide identity and genomic DNA-DNA hybridization values. These metrics confirm that isolates SL13 and SL54 belong to a novel species that is most closely related to NBRC 115203 and that these strains together with DSM 41836, DSM 42083 belong to the genus . Consequently, it is proposed that the isolates be recognized as a new species, comb. nov., with isolate SL54 (=DSM 111111=PCM3044) as the type strain, and that , and be transferred to the genus as comb. nov., comb. nov. and comb. nov. Emended descriptions are given for the genus , the family and for which was found to be a close relative of the isolates in the 16S rRNA gene sequence analyses. It is also proposed that be transferred to the genus as comb. nov based on its position in the MLSA and phylogenomic trees and associated genomic data.
从松树落叶中分离出丝状放线菌，命名为SL13和SL54，并采用多相分类法确定其分类地位。这些分离株表现出化学分类学和形态学特征，与其在该科中的分类一致。它们形成广泛分支的基内菌丝，带有气生菌丝，气生菌丝分化为圆柱形孢子的直链。全细胞水解产物含有ll-二氨基庚二酸、葡萄糖、甘露糖和核糖，主要的异戊二烯醌是MK-9(H)，极性脂质是双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰甘油和糖磷脂，主要脂肪酸是anteiso-C、iso-C、iso-C和anteiso-C。基于16S rRNA基因序列和保守管家基因的多位点基因序列构建的系统发育树表明，这些分离株形成了一个得到充分支持的谱系，与NBRC 115203关系最为密切。在多位点序列分析树中，所有这些菌株与DSM 46488、DSM 41836和DSM 42083一起形成了一个明确的分支。分离株的基因组草图组装富含预测可产生新型特殊代谢产物的生物合成基因簇和与应激相关的基因，这有助于深入了解它们如何适应松树落叶中普遍存在的恶劣条件。从系统基因组学角度来看，这两个分离株与、、和的模式菌株属于同一谱系；这些关系通过高平均氨基酸同一性、平均核苷酸同一性和基因组DNA-DNA杂交值得到证实。这些指标证实，分离株SL13和SL54属于一个与NBRC 115203关系最为密切的新物种，并且这些菌株与DSM 41836、DSM 42083一起属于属。因此，建议将这些分离株认定为一个新物种，新组合，以分离株SL54（=DSM 111111=PCM3044）作为模式菌株，并将、和转移到属，新组合、新组合和新组合。对属、科以及在16S rRNA基因序列分析中被发现是这些分离株近亲的进行了修订描述。还建议根据其在MLSA和系统基因组树中的位置以及相关基因组数据，将转移到属，新组合。
