Cui Shun-Qiang, Liang Xin-Yi, Cai Zhi-Qiang, Yuan Hou-Qun, Zhang Qiu-Yu, Xia Yi-Fan, Sandra A K I M A N A, Guo Ling-Yi, Wang Ran, Zhao Shi-Yu, Li Yan-Xia, Bao Guang-Ming
Key Laboratory of Fermentation Engineering (Ministry of Education), National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Hubei Key Laboratory of Industrial Microbiology, School of Life and Health Sciences, Hubei University of Technology, Wuhan 430068, China.
Key Laboratory of Fermentation Engineering (Ministry of Education), National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Hubei Key Laboratory of Industrial Microbiology, School of Life and Health Sciences, Hubei University of Technology, Wuhan 430068, China.
Food Chem. 2025 Oct 30;490:145116. doi: 10.1016/j.foodchem.2025.145116. Epub 2025 Jun 11.
Chronic exposure to oxidants such as benzoyl peroxide (BPO) and hypochlorite (ClO) poses significant health risks, driving the urgent need for sensitive detection methods. We developed a dual-emitting ratiometric fluorescence probe based on europium metal-organic frameworks encapsulated nitrogen-doped carbon dots (N-CDs@Eu-MOFs). This probe enables rapid detection within 1 min for BPO and 3 min for ClO, demonstrating broad linear ranges (0.1-6.0 μM) with ultralow detection limits (6.80 nM for BPO, 5.78 nM for ClO). N-CDs@Eu-MOFs successfully quantified both analytes in five real food matrices, showing recovery rates of 91.94-108.33 % for BPO and 91.25-107.22 % for ClO, confirming its reliability in real-world applications. Additionally, portable N-CDs@Eu-MOFs-embedded gel beads were fabricated that facilitate smartphone-based BPO quantification through RGB analysis, achieving recovery rates of 91.41-105.44 % in food samples. This instrument-free approach combines laboratory-grade accuracy with on-site practicality, significantly advancing food safety monitoring.
长期暴露于过氧苯甲酰（BPO）和次氯酸盐（ClO）等氧化剂会带来重大健康风险，这促使人们迫切需要灵敏的检测方法。我们开发了一种基于铕金属有机框架包裹氮掺杂碳点（N-CDs@Eu-MOFs）的双发射比率荧光探针。该探针能够在1分钟内快速检测BPO，3分钟内快速检测ClO，具有较宽的线性范围（0.1 - 6.0 μM）和超低检测限（BPO为6.80 nM，ClO为5.78 nM）。N-CDs@Eu-MOFs成功地对五种真实食品基质中的两种分析物进行了定量，BPO的回收率为91.94 - 108.33%，ClO的回收率为91.25 - 107.22%，证实了其在实际应用中的可靠性。此外，还制备了嵌入N-CDs@Eu-MOFs的便携式凝胶珠，通过RGB分析实现了基于智能手机的BPO定量，在食品样品中的回收率为91.41 - 105.44%。这种无需仪器的方法将实验室级别的准确性与现场实用性相结合，显著推进了食品安全监测。
