Cheng H, Jacobs P M, McPherson K, Noble M J
Arch Ophthalmol. 1985 Oct;103(10):1478-81. doi: 10.1001/archopht.1985.01050100054017.
The precision of estimating endothelial cell density of the cornea, using a noncontact method of specular microscopy, was assessed by asking eight individuals with known densities in one operated-on and one unoperated-on eye to have photography on two occasions in the same day. Three photographs of each eye were assessed by two individuals using masking procedures. Interobserver differences were negligible and estimates were similar except for one eye with low density values. The method was applied to a natural history study in which 103 eyes had cell-density estimates at zero and two years, and mean cell loss was found to be 2%. A collateral study using only good photographs that were available at both zero and two years showed a similar loss of 1.86% and reduced the number of counts showing unexpectedly high gains and losses.
通过让8名个体（其一只手术眼和一只未手术眼的角膜内皮细胞密度已知）在同一天进行两次摄影，评估了使用非接触式镜面显微镜法估计角膜内皮细胞密度的精度。两名观察者采用遮盖程序对每只眼睛的三张照片进行评估。观察者间差异可忽略不计，除了一只密度值较低的眼睛外，估计值相似。该方法应用于一项自然史研究，其中103只眼睛在零年和两年时进行了细胞密度估计，发现平均细胞损失率为2%。一项仅使用零年和两年时均可用的优质照片的附带研究显示，细胞损失率相似，为1.86%，并减少了显示意外高增益和损失的计数数量。
