Souza João Gabriel S, Bertolini Martinna, Liu Jett, Nagay Bruna Egumi, Martins Rodrigo, Costa Raphael C, Brunson Jason Cory, Shibli Jamil, Figueiredo Luciene Cristina, Dongari-Bagtzoglou Anna, Feres Magda, Barão Valentim Adelino Ricardo, Bor Batbileg
Dental Research Division, Universidade Universus Veritas Guarulhos, Guarulhos, São Paulo 07023-070, Brazil.
Department of Prosthodontics and Periodontology, Piracicaba Dental School, Universidade Estadual de Campinas (UNICAMP), Piracicaba, São Paulo 13414-903, Brazil.
ACS Nano. 2025 Jul 1;19(25):23393-23413. doi: 10.1021/acsnano.5c06573. Epub 2025 Jun 15.
The oral environment is composed of a diverse array of proteins, and any substrate inserted into this habitat promptly becomes subjected to protein adsorption and bacterial colonization. However, the predictive and modulatory nature of implant surfaces coated with salivary pellicle proteomes in microbial adhesion has not been explored using high-throughput techniques. Thus, using human saliva for salivary pellicle adsorption and microbial accumulation, we compared adsorption and community formation on titanium (Ti) biomaterials (implant devices) and dental surfaces (enamel and dentine). The proteomic profile was evaluated by liquid chromatography coupled with tandem mass spectrometry, and the microbiome was assessed using 16S RNA sequencing. Linear discriminant analysis (LDA) and canonical correlation analysis (CCA) were used to quantify variation in analyte amounts and identify likely biomarkers. Substrates were analyzed regarding their physical, chemical, and topographical properties. Our results showed that the salivary pellicle proteomes on Ti exhibited differences in composition and protein intensities compared with dental surfaces. These differences in proteomes affected the biological processes at the level of microbiome accumulation. Geometric analysis showed greater similarity between Ti and enamel proteomes, while dentine differed markedly. Ti harbors a microbiome community that differs from that of dental surfaces. Canonical correlation analysis (CCA) pinpointed proteins that promoted or inhibited the adherence of specific microbes. Apolipoprotein E showed a strong negative correlation (>0.8) with . Higher levels of the protein on dental surfaces were associated with reduced microbial adhesion, whereas its absence on Ti surfaces facilitated increased bacterial adhesion. These findings provide valuable insights into the initial biological responses after the insertion of implanted devices, which can be leveraged by biomedical engineering to develop biomaterials with enhanced outcomes and prevent microbial accumulation.
口腔环境由多种蛋白质组成，任何植入该环境的底物都会迅速受到蛋白质吸附和细菌定植的影响。然而，尚未使用高通量技术探索涂有唾液薄膜蛋白质组的植入物表面在微生物黏附中的预测性和调节性。因此，我们利用人类唾液进行唾液薄膜吸附和微生物积累实验，比较了钛（Ti）生物材料（植入装置）和牙齿表面（牙釉质和牙本质）上的吸附和群落形成情况。通过液相色谱-串联质谱联用技术评估蛋白质组学特征，并使用16S RNA测序评估微生物群落。采用线性判别分析（LDA）和典型相关分析（CCA）来量化分析物含量的变化并识别可能的生物标志物。对底物的物理、化学和形貌特性进行了分析。我们的结果表明，与牙齿表面相比，Ti上的唾液薄膜蛋白质组在组成和蛋白质强度上存在差异。这些蛋白质组差异影响了微生物积累水平的生物学过程。几何分析表明，Ti和牙釉质蛋白质组之间的相似性更大，而牙本质则明显不同。Ti上的微生物群落与牙齿表面的不同。典型相关分析（CCA）确定了促进或抑制特定微生物黏附的蛋白质。载脂蛋白E与……呈强烈负相关（>0.8）。牙齿表面该蛋白质水平较高与微生物黏附减少有关，而Ti表面缺乏该蛋白质则促进细菌黏附增加。这些发现为植入装置植入后的初始生物学反应提供了有价值的见解，生物医学工程可利用这些见解开发具有更好效果的生物材料并防止微生物积累。
