Division of Biology, Chemistry, and Materials Science; Office of Science and Engineering Laboratories; Center for Devices and Radiological Health, US Food and Drug Administration, Silver Spring, Maryland, USA.
J Biomed Mater Res A. 2024 Mar;112(3):373-389. doi: 10.1002/jbm.a.37635. Epub 2023 Oct 30.
Biomaterials with nanoscale topography have been increasingly investigated for medical device applications to improve tissue-material interactions. This study assessed the impact of nanoengineered titanium surface domain sizes on early biological responses that can significantly affect tissue interactions. Nanostructured titanium coatings with distinct nanoscale surface roughness were deposited on quartz crystal microbalance with dissipation (QCM-D) sensors by physical vapor deposition. Physico-chemical characterization was conducted to assess nanoscale surface roughness, nano-topographical morphology, wettability, and atomic composition. The results demonstrated increased projected surface area and hydrophilicity with increasing nanoscale surface roughness. The adsorption properties of albumin and fibrinogen, two major plasma proteins that readily encounter implanted surfaces, on the nanostructured surfaces were measured using QCM-D. Significant differences in the amounts and viscoelastic properties of adsorbed proteins were observed, dependent on the surface roughness, protein type, protein concentration, and protein binding affinity. The impact of protein adsorption on subsequent biological responses was also examined using qualitative and quantitative in vitro evaluation of human platelet adhesion, aggregation, and activation. Qualitative platelet morphology assessment indicated increased platelet activation/aggregation on titanium surfaces with increased roughness. These data suggest that nanoscale differences in titanium surface roughness influence biological responses that may affect implant integration.
具有纳米形貌的生物材料越来越多地被用于医疗器械应用中，以改善组织-材料相互作用。本研究评估了纳米工程钛表面域大小对早期生物学反应的影响，这些反应可能会显著影响组织相互作用。通过物理气相沉积在石英晶体微天平（QCM-D）传感器上沉积了具有不同纳米级表面粗糙度的纳米结构钛涂层。通过物理化学特性评估了纳米级表面粗糙度、纳米形貌、润湿性和原子组成。结果表明，随着纳米级表面粗糙度的增加，表面的投影面积和亲水性增加。使用 QCM-D 测量了两种主要的血浆蛋白白蛋白和纤维蛋白原在纳米结构表面上的吸附特性。发现吸附蛋白的量和粘弹性特性存在显著差异，这取决于表面粗糙度、蛋白质类型、蛋白质浓度和蛋白质结合亲和力。还通过体外定性和定量评估人血小板黏附、聚集和激活来研究蛋白质吸附对随后的生物学反应的影响。血小板形态学评估表明，随着粗糙度的增加，钛表面上的血小板活化/聚集增加。这些数据表明，钛表面粗糙度的纳米差异会影响可能影响植入物整合的生物学反应。
